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| | <StructureSection load='6o6m' size='340' side='right'caption='[[6o6m]], [[Resolution|resolution]] 2.51Å' scene=''> | | <StructureSection load='6o6m' size='340' side='right'caption='[[6o6m]], [[Resolution|resolution]] 2.51Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6o6m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chltf Chltf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O6M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6O6M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6o6m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloracidobacterium_thermophilum_B Chloracidobacterium thermophilum B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O6M FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.506Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cabther_A1318 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=981222 CHLTF])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o6m OCA], [http://pdbe.org/6o6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o6m RCSB], [http://www.ebi.ac.uk/pdbsum/6o6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o6m ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o6m OCA], [https://pdbe.org/6o6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o6m RCSB], [https://www.ebi.ac.uk/pdbsum/6o6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o6m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/G2LET6_CHLTF G2LET6_CHLTF] |
| - | Ergothioneine is a thiohistidine derivative with potential benefits on many aging-related diseases. The central step of aerobic ergothioneine biosynthesis is the oxidative C-S bond formation reaction catalyzed by mononuclear nonheme iron sulfoxide synthases (EgtB and Egt1). Thus far, only the Mycobacterium thermoresistibile EgtB (EgtB Mth ) crystal structure is available, while the structural information for the more industrially attractive Egt1 enzyme is not. Herein, we reported the crystal structure of the ergothioneine sulfoxide synthase (EgtB Cth ) from Candidatus Chloracidobacterium thermophilum. EgtB Cth has both EgtB- and Egt1-type of activities. Guided by the structural information, we conducted Rosetta Enzyme Design calculations, and we biochemically demonstrated that EgtB Cth can be engineered more toward Egt1-type of activity. This study provides information regarding the factors governing the substrate selectivity in Egt1- and EgtB-catalysis and lays the groundwork for future sulfoxide synthase engineering toward the development of an effective ergothioneine process through a synthetic biology approach.
| + | |
| - | | + | |
| - | Crystal Structure of the Ergothioneine Sulfoxide Synthase from Candidatus Chloracidobacterium thermophilum and Structure-Guided Engineering To Modulate Its Substrate Selectivity.,Naowarojna N, Irani S, Hu W, Cheng R, Zhang L, Li X, Chen J, Zhang YJ, Liu P ACS Catal. 2019 Aug 2;9(8):6955-6961. doi: 10.1021/acscatal.9b02054. Epub 2019, Jul 2. PMID:32257583<ref>PMID:32257583</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6o6m" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chltf]] | + | [[Category: Chloracidobacterium thermophilum B]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Irani, S]] | + | [[Category: Irani S]] |
| - | [[Category: Zhang, Y]] | + | [[Category: Zhang Y]] |
| - | [[Category: Ergothioneine]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Non-heme iron dependent enzyme]]
| + | |
