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1sjj

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Revision as of 07:25, 27 January 2022

Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin

Structural highlights

1sjj is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1hci, 1aoa, 1dxx, 1qag, 1cfd
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACTN1_CHICK] F-actin cross-linking protein is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation.

A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region.,Liu J, Taylor DW, Taylor KA J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:15050827^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu J, Taylor DW, Taylor KA. A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region. J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:15050827 doi:10.1016/j.jmb.2004.02.034

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sjj"

@@ Line 3: / Line 3: @@
 <StructureSection load='1sjj' size='340' side='right'caption='[[1sjj]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1sjj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SJJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1sjj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJJ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hci|1hci]], [[1aoa|1aoa]], [[1dxx|1dxx]], [[1qag|1qag]], [[1cfd|1cfd]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hci|1hci]], [[1aoa|1aoa]], [[1dxx|1dxx]], [[1qag|1qag]], [[1cfd|1cfd]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjj OCA], [http://pdbe.org/1sjj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sjj RCSB], [http://www.ebi.ac.uk/pdbsum/1sjj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjj OCA], [https://pdbe.org/1sjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjj RCSB], [https://www.ebi.ac.uk/pdbsum/1sjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ACTN1_CHICK ACTN1_CHICK]] F-actin cross-linking protein is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
+[[https://www.uniprot.org/uniprot/ACTN1_CHICK ACTN1_CHICK]] F-actin cross-linking protein is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1sjj

From Proteopedia

Revision as of 07:25, 27 January 2022

Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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