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| | <StructureSection load='5ef9' size='340' side='right'caption='[[5ef9]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='5ef9' size='340' side='right'caption='[[5ef9]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ef9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Inble Inble]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EF9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EF9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ef9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus_(B/Lee/1940) Influenza B virus (B/Lee/1940)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EF9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EF9 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5efa|5efa]], [[5efc|5efc]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=518987 INBLE])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ef9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ef9 OCA], [https://pdbe.org/5ef9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ef9 RCSB], [https://www.ebi.ac.uk/pdbsum/5ef9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ef9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ef9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ef9 OCA], [http://pdbe.org/5ef9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ef9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ef9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ef9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PB2_INBLE PB2_INBLE]] Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity). | + | [https://www.uniprot.org/uniprot/PB2_INBLE PB2_INBLE] Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Inble]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ma, X]] | + | [[Category: Ma X]] |
| - | [[Category: Shia, S]] | + | [[Category: Shia S]] |
| - | [[Category: Cap-binding domain]]
| + | |
| - | [[Category: Protein binding]]
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| Structural highlights
Function
PB2_INBLE Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity).
Publication Abstract from PubMed
Influenza virus polymerase catalyzes the transcription of viral mRNAs by a process known as 'cap-snatching', where the 5'-cap of cellular pre-mRNA is recognized by the PB2 subunit and cleaved 10-13 nucleotides downstream of the cap by the endonuclease PA subunit. Although this mechanism is common to both influenza A (FluA) and B (FluB) viruses, FluB PB2 recognizes a wider range of cap structures including m7GpppGm-, m7GpppG-, and GpppG-RNA, while FluA PB2 utilizes methylated G-capped RNA specifically. Biophysical studies with isolated PB2 cap-binding domain (PB2cap) confirm that FluB PB2 has expanded mRNA cap recognition capability although the affinities towards m7GTP are significantly reduced when compared to FluA PB2. The X-ray co-structures of the FluB PB2cap with bound cap analogs m7GTP and GTP reveal an inverted GTP binding mode that is distinct from the cognate m7GTP binding mode shared between FluA and FluB PB2. These results delineate the commonalities and differences in the cap-binding site between FluA and FluB PB2 and will aid structure-guided drug design efforts to identify dual inhibitors of both FluA and FluB PB2.
Molecular basis of mRNA cap recognition by Influenza B polymerase PB2 subunit.,Xie L, Wartchow C, Shia S, Uehara K, Steffek M, Warne R, Sutton J, Muiru GT, Leonard VH, Bussiere DE, Ma X J Biol Chem. 2015 Nov 11. pii: jbc.M115.693051. PMID:26559973[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Xie L, Wartchow C, Shia S, Uehara K, Steffek M, Warne R, Sutton J, Muiru GT, Leonard VH, Bussiere DE, Ma X. Molecular basis of mRNA cap recognition by Influenza B polymerase PB2 subunit. J Biol Chem. 2015 Nov 11. pii: jbc.M115.693051. PMID:26559973 doi:http://dx.doi.org/10.1074/jbc.M115.693051
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