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5eg1

From Proteopedia

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Antibacterial peptide ABC transporter McjD with a resolved lipid

Structural highlights

5eg1 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.42Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MCJD_ECOLX Is able to protect a cell, which harbors the plasmid pTUC100 encoding microcin J25, against microcin J25. Is required for microcin J25 export out of the producing cells.^[1] ^[2]

Publication Abstract from PubMed

The lipid bilayer is a dynamic environment that consists of a mixture of lipids with different properties that regulate the function of membrane proteins; these lipids are either annular, masking the protein hydrophobic surface, or specific lipids, essential for protein function. In this study, using tandem mass spectrometry, we have identified specific lipids associated with the Escherichia coli ABC transporter McjD, which translocates the antibacterial peptide MccJ25. Using non-denaturing mass spectrometry, we show that McjD in complex with MccJ25 survives the gas-phase. Partial delipidation of McjD resulted in reduced ATPase activity and thermostability as shown by Circular Dichroism, both of which could be restored upon addition of defined E. coli lipids. We have resolved a phosphatidylglycerol lipid associated with McjD at 3.4 A resolution, while molecular dynamic simulations carried out in different lipid environments assessed the binding of specific lipids to McjD. Combined, our data show a synergistic effect of zwitterionic and negatively charged lipids on the activity of McjD; the zwitterionic lipids provide structural stability to McjD whereas the negatively charged lipids are essential for its function.

Structural and functional basis for lipid synergy on the activity of the antibacterial peptide ABC transporter McjD.,Mehmood S, Corradi V, Choudhury HG, Hussain R, Becker P, Axford D, Zirah S, Rebuffat S, Tieleman DP, Robinson CV, Beis K J Biol Chem. 2016 Aug 23. pii: jbc.M116.732107. PMID:27555327^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Solbiati JO, Ciaccio M, Farias RN, Gonzalez-Pastor JE, Moreno F, Salomon RA. Sequence analysis of the four plasmid genes required to produce the circular peptide antibiotic microcin J25. J Bacteriol. 1999 Apr;181(8):2659-62. PMID:10198038
↑ Solbiati JO, Ciaccio M, Farias RN, Salomon RA. Genetic analysis of plasmid determinants for microcin J25 production and immunity. J Bacteriol. 1996 Jun;178(12):3661-3. PMID:8655570
↑ Mehmood S, Corradi V, Choudhury HG, Hussain R, Becker P, Axford D, Zirah S, Rebuffat S, Tieleman DP, Robinson CV, Beis K. Structural and functional basis for lipid synergy on the activity of the antibacterial peptide ABC transporter McjD. J Biol Chem. 2016 Aug 23. pii: jbc.M116.732107. PMID:27555327 doi:http://dx.doi.org/10.1074/jbc.M116.732107

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5eg1"

Categories: Escherichia coli | Large Structures | Beis K | Choudhury HG

@@ Line 3: / Line 3: @@
 <StructureSection load='5eg1' size='340' side='right'caption='[[5eg1]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5eg1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EG1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EG1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5eg1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EG1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P6L:(2S)-3-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-2-[(6E)-HEXADEC-6-ENOYLOXY]PROPYL+(8E)-OCTADEC-8-ENOATE'>P6L</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.42&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pl0|4pl0]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=P6L:(2S)-3-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-2-[(6E)-HEXADEC-6-ENOYLOXY]PROPYL+(8E)-OCTADEC-8-ENOATE'>P6L</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mcjD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eg1 OCA], [https://pdbe.org/5eg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eg1 RCSB], [https://www.ebi.ac.uk/pdbsum/5eg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eg1 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eg1 OCA], [http://pdbe.org/5eg1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eg1 RCSB], [http://www.ebi.ac.uk/pdbsum/5eg1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eg1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MCJD_ECOLX MCJD_ECOLX]] Is able to protect a cell, which harbors the plasmid pTUC100 encoding microcin J25, against microcin J25. Is required for microcin J25 export out of the producing cells.<ref>PMID:10198038</ref> <ref>PMID:8655570</ref>
+[https://www.uniprot.org/uniprot/MCJD_ECOLX MCJD_ECOLX] Is able to protect a cell, which harbors the plasmid pTUC100 encoding microcin J25, against microcin J25. Is required for microcin J25 export out of the producing cells.<ref>PMID:10198038</ref> <ref>PMID:8655570</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Beis, K]]
+[[Category: Beis K]]
-[[Category: Choudhury, H G]]
+[[Category: Choudhury HG]]
-[[Category: Abc transporter]]
-[[Category: Lipid]]
-[[Category: Membrane protein]]
-[[Category: Transport protein]]

5eg1

From Proteopedia

Current revision

Antibacterial peptide ABC transporter McjD with a resolved lipid

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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