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1b5e

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{{STRUCTURE_1b5e| PDB=1b5e | SCENE= }}
{{STRUCTURE_1b5e| PDB=1b5e | SCENE= }}
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'''DCMP HYDROXYMETHYLASE FROM T4'''
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===DCMP HYDROXYMETHYLASE FROM T4===
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==Overview==
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Bacteriophage T4 deoxycytidylate hydroxymethylase (EC 2.1.2.8), a homodimer of 246-residue subunits, catalyzes hydroxymethylation of the cytosine base in deoxycytidylate (dCMP) to produce 5-hydroxymethyl-dCMP. It forms part of a phage DNA protection system and appears to function in vivo as a component of a multienzyme complex called deoxyribonucleoside triphosphate (dNTP) synthetase. We have determined its crystal structure in the presence of the substrate dCMP at 1.6 A resolution. The structure reveals a subunit fold and a dimerization pattern in common with thymidylate synthases, despite low (approximately 20%) sequence identity. Among the residues that form the dCMP binding site, those interacting with the sugar and phosphate are arranged in a configuration similar to the deoxyuridylate binding site of thymidylate synthases. However, the residues interacting directly or indirectly with the cytosine base show a more divergent structure and the presumed folate cofactor binding site is more open. Our structure reveals a water molecule properly positioned near C-6 of cytosine to add to the C-7 methylene intermediate during the last step of hydroxymethylation. On the basis of sequence comparison and crystal packing analysis, a hypothetical model for the interaction between T4 deoxycytidylate hydroxymethylase and T4 thymidylate synthase in the dNTP-synthesizing complex has been built.
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{{ABSTRACT_PUBMED_10064578}}
==About this Structure==
==About this Structure==
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[[Category: Dntp synthesizing complex]]
[[Category: Dntp synthesizing complex]]
[[Category: Hydroxymethylase]]
[[Category: Hydroxymethylase]]
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Revision as of 15:18, 30 June 2008

Image:1b5e.png

Template:STRUCTURE 1b5e

DCMP HYDROXYMETHYLASE FROM T4

Template:ABSTRACT PUBMED 10064578

About this Structure

1B5E is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonucleoside triphosphate-synthesizing complex., Song HK, Sohn SH, Suh SW, EMBO J. 1999 Mar 1;18(5):1104-13. PMID:10064578

Page seeded by OCA on Mon Jun 30 18:18:16 2008

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