6m58
From Proteopedia
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==Crystal structure of a complex between human serum albumin and the antibody Fab SL335== | ==Crystal structure of a complex between human serum albumin and the antibody Fab SL335== | ||
| - | <StructureSection load='6m58' size='340' side='right'caption='[[6m58]]' scene=''> | + | <StructureSection load='6m58' size='340' side='right'caption='[[6m58]], [[Resolution|resolution]] 2.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M58 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M58 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m58]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M58 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M58 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m58 OCA], [http://pdbe.org/6m58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m58 RCSB], [http://www.ebi.ac.uk/pdbsum/6m58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m58 ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m58 OCA], [http://pdbe.org/6m58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m58 RCSB], [http://www.ebi.ac.uk/pdbsum/6m58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m58 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Disease == | ||
| + | [[http://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[http://omim.org/entry/103600 103600]]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human serum albumin (HSA) has been used to extend the serum half-lives of various protein therapeutics through genetic fusion because HSA exhibits an exceptionally long circulation time as a result of neonatal Fc receptor (FcRn)-mediated recycling. As another serum half-life extender, the human antibody Fab SL335 that strongly binds HSA was developed. When SL335 was fused to a protein therapeutic, SL335 was shown to prolong the half-life of the drug. Despite the significance of SL335-HSA binding in the extension of drug circulation time, it remains unclear how SL335 interacts with HSA at a molecular structural level. To reveal the structural basis of HSA recognition by SL335, we determined the crystal structure of the SL335-HSA complex at a resolution of 2.95 A. SL335 binds HSA at a 1:1 stoichiometry. SL335 uses the exposed loops of its heavy and light chains to specifically recognize the IIa and IIb subdomains of HSA. The SL335 epitope is located on the opposite side of the FcRn-binding site and does not overlap with it, suggesting that SL335 extends the serum half-lives of itself and its fusion partner through an FcRn-dependent recycling mechanism. | ||
| + | |||
| + | Structural basis of serum albumin recognition by SL335, an antibody Fab extending the serum half-life of protein therapeutics.,Cho SY, Han J, Cha SH, Yoon SI Biochem Biophys Res Commun. 2020 Apr 10. pii: S0006-291X(20)30624-0. doi:, 10.1016/j.bbrc.2020.03.133. PMID:32284170<ref>PMID:32284170</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6m58" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cho | + | [[Category: Cho, S Y]] |
| - | [[Category: Yoon | + | [[Category: Yoon, S I]] |
| + | [[Category: Anti-albumin]] | ||
| + | [[Category: Antibody fab]] | ||
| + | [[Category: Immune system]] | ||
| + | [[Category: Serum albumin]] | ||
Revision as of 07:18, 27 May 2020
Crystal structure of a complex between human serum albumin and the antibody Fab SL335
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