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| | <StructureSection load='6jp2' size='340' side='right'caption='[[6jp2]], [[Resolution|resolution]] 2.27Å' scene=''> | | <StructureSection load='6jp2' size='340' side='right'caption='[[6jp2]], [[Resolution|resolution]] 2.27Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jp2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"candidatus_methanomethylophilus_alvus"_borrel_et_al._2012 "candidatus methanomethylophilus alvus" borrel et al. 2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JP2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JP2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jp2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Methanomethylophilus_alvus Candidatus Methanomethylophilus alvus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JP2 FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.272Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jp2 OCA], [http://pdbe.org/6jp2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jp2 RCSB], [http://www.ebi.ac.uk/pdbsum/6jp2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jp2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jp2 OCA], [https://pdbe.org/6jp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jp2 RCSB], [https://www.ebi.ac.uk/pdbsum/6jp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jp2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/M9SC49_METAX M9SC49_METAX] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 16: |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6jp2" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6jp2" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Candidatus methanomethylophilus alvus borrel et al. 2012]] | + | [[Category: Candidatus Methanomethylophilus alvus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kuratani, M]] | + | [[Category: Kuratani M]] |
| - | [[Category: Yanagisawa, T]] | + | [[Category: Yanagisawa T]] |
| - | [[Category: Yokoyama, S]] | + | [[Category: Yokoyama S]] |
| - | [[Category: Aminoacyl-trna synthetase]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Non-natural amino acid]]
| + | |
| - | [[Category: Pyrrolysyl-trna synthetase]]
| + | |
| - | [[Category: Translation]]
| + | |
| - | [[Category: Trna]]
| + | |
| Structural highlights
Function
M9SC49_METAX
Publication Abstract from PubMed
Pyrrolysyl-tRNA synthetase (PylRS)/tRNA(Pyl) pairs from Methanosarcina mazei and Methanosarcina barkeri are widely used for site-specific incorporations of non-canonical amino acids into proteins (genetic code expansion). In this study, we achieved the full productivity of cell-free protein synthesis for difficult, bulky non-canonical amino acids, such as N(epsilon)-((((E)-cyclooct-2-en-1-yl)oxy)carbonyl)-l-lysine (TCO*Lys), by using Methanomethylophilus alvus PylRS. First, based on the crystal structure of M. alvus PylRS, the productivities for various non-canonical amino acids were greatly increased by rational engineering of the amino acid-binding pocket. The productivities were further enhanced by using a much higher concentration of PylRS over that of M. mazei PylRS, or by mutating the outer layer of the amino acid-binding pocket. Thus, we achieved full productivity even for TCO*Lys. The quantity and quality of the cell-free-produced antibody fragment containing TCO*Lys were drastically improved. These results demonstrate the importance of full productivity for the expanded genetic code.
Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase.,Seki E, Yanagisawa T, Kuratani M, Sakamoto K, Yokoyama S ACS Synth Biol. 2020 Apr 17;9(4):718-732. doi: 10.1021/acssynbio.9b00288. Epub, 2020 Mar 17. PMID:32182048[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Seki E, Yanagisawa T, Kuratani M, Sakamoto K, Yokoyama S. Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase. ACS Synth Biol. 2020 Apr 17;9(4):718-732. doi: 10.1021/acssynbio.9b00288. Epub, 2020 Mar 17. PMID:32182048 doi:http://dx.doi.org/10.1021/acssynbio.9b00288
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