1b75

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[[Image:1b75.gif|left|200px]]
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{{STRUCTURE_1b75| PDB=1b75 | SCENE= }}
{{STRUCTURE_1b75| PDB=1b75 | SCENE= }}
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'''SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI'''
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===SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI===
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==Overview==
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The structure of the Escherichia coli ribosomal protein L25 has been determined to an r.m.s. displacement of backbone heavy atoms of 0.62 +/- 0.14 A by multi-dimensional heteronuclear NMR spectroscopy on protein samples uniformly labeled with 15N or 15N/13C. L25 shows a new topology for RNA-binding proteins consisting of a six-stranded beta-barrel and two alpha-helices. A putative RNA-binding surface for L25 has been obtained by comparison of backbone 15N chemical shifts for L25 with and without a bound cognate RNA containing the eubacterial E-loop that is the site for binding of L25 to 5S ribosomal RNA. Sequence comparisons with related proteins, including the general stress protein, CTC, show that the residues involved in RNA binding are highly conserved, thereby providing further confirmation of the binding surface. Tertiary structure comparisons indicate that the six-stranded beta-barrels of L25 and of the tRNA anticodon-binding domain of glutaminyl-tRNA synthetase are similar.
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(as it appears on PubMed at http://www.pubmed.gov), where 9799245 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9799245}}
==About this Structure==
==About this Structure==
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1B75 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B75 OCA].
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1B75 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B75 OCA].
==Reference==
==Reference==
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[[Category: Ribosomal protein]]
[[Category: Ribosomal protein]]
[[Category: Rna-binding protein]]
[[Category: Rna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:09:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:25:43 2008''

Revision as of 15:25, 30 June 2008

Image:1b75.png

Template:STRUCTURE 1b75

SOLUTION STRUCTURE OF RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 9799245

About this Structure

1B75 is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases., Stoldt M, Wohnert J, Gorlach M, Brown LR, EMBO J. 1998 Nov 2;17(21):6377-84. PMID:9799245

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