|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5f7p' size='340' side='right'caption='[[5f7p]], [[Resolution|resolution]] 2.84Å' scene=''> | | <StructureSection load='5f7p' size='340' side='right'caption='[[5f7p]], [[Resolution|resolution]] 2.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5f7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismo Lismo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F7P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5F7P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5f7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F7P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.84Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f7q|5f7q]], [[5f7r|5f7r]], [[5f7v|5f7v]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmo0178 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f7p OCA], [https://pdbe.org/5f7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f7p RCSB], [https://www.ebi.ac.uk/pdbsum/5f7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f7p ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5f7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f7p OCA], [http://pdbe.org/5f7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f7p RCSB], [http://www.ebi.ac.uk/pdbsum/5f7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f7p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8YAF1_LISMO Q8YAF1_LISMO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lismo]] | + | [[Category: Listeria monocytogenes EGD-e]] |
| - | [[Category: Anderson, W F]] | + | [[Category: Anderson WF]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Light SH]] |
| - | [[Category: Light, S H]] | + | |
| - | [[Category: Csgid]]
| + | |
| - | [[Category: Kinase]]
| + | |
| - | [[Category: Open reading frame]]
| + | |
| - | [[Category: Repressor]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
Q8YAF1_LISMO
Publication Abstract from PubMed
Here we employ a 'systems structural biology' approach to functionally characterize an unconventional alpha-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.
Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis.,Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF Nat Microbiol. 2016 Nov 7;2:16202. doi: 10.1038/nmicrobiol.2016.202. PMID:27819654[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF. Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis. Nat Microbiol. 2016 Nov 7;2:16202. doi: 10.1038/nmicrobiol.2016.202. PMID:27819654 doi:http://dx.doi.org/10.1038/nmicrobiol.2016.202
|
