|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of Aedes aegypti OBP22 in the complex with arachidonic acid== | | ==Structure of Aedes aegypti OBP22 in the complex with arachidonic acid== |
| - | <StructureSection load='6nbn' size='340' side='right'caption='[[6nbn]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | + | <StructureSection load='6nbn' size='340' side='right'caption='[[6nbn]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nbn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aedae Aedae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NBN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nbn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NBN FirstGlance]. <br> |
| | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACD:ARACHIDONIC+ACID'>ACD</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACD:ARACHIDONIC+ACID'>ACD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">5567053, AAEL005772 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7159 AEDAE])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbn OCA], [https://pdbe.org/6nbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nbn RCSB], [https://www.ebi.ac.uk/pdbsum/6nbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbn ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbn OCA], [http://pdbe.org/6nbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nbn RCSB], [http://www.ebi.ac.uk/pdbsum/6nbn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbn ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q1HRL7_AEDAE Q1HRL7_AEDAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 24: |
Line 25: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aedae]] | + | [[Category: Aedes aegypti]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jones, D N]] | + | [[Category: Jones DN]] |
| - | [[Category: Wang, J]] | + | [[Category: Wang J]] |
| - | [[Category: Odorant-binding protein chemo-sensory signaling lipid binding]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
Q1HRL7_AEDAE
Publication Abstract from PubMed
Aedes aegypti is the primary vector for transmission of Dengue, Zika and chikungunya viruses. Previously it was shown that Dengue virus infection of the mosquito led to an in increased expression of the odorant binding protein 22 (AeOBP22) within the mosquito salivary gland and that siRNA mediated knockdown of AeOBP22 led to reduced mosquito feeding behaviors. Insect OBPs are implicated in the perception, storage and transport of chemosensory signaling molecules including air-borne odorants and pheromones. AeOBP22 is unusual as it is additionally expressed in multiple tissues, including the antenna, the male reproductive glands and is transferred to females during reproduction, indicating multiple roles in the mosquito life cycle. However, it is unclear what role it plays in these tissues and what ligands it interacts with. Here we present solution and X-ray crystallographic studies that indicate a potential role of AeOBP22 binding to fatty acids, and that the specificity for longer chain fatty acids is regulated by a conformational change in the C-terminal tail that leads to creation of an enlarged binding cavity that enhances binding affinity. This study sheds light onto the native ligands for AeOBP22 and provides insight into its potential functions in different tissues.
Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail.,Wang J, Murphy EJ, Nix JC, Jones DNM Sci Rep. 2020 Feb 24;10(1):3300. doi: 10.1038/s41598-020-60242-9. PMID:32094450[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wang J, Murphy EJ, Nix JC, Jones DNM. Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail. Sci Rep. 2020 Feb 24;10(1):3300. doi: 10.1038/s41598-020-60242-9. PMID:32094450 doi:http://dx.doi.org/10.1038/s41598-020-60242-9
|
