6w9q
From Proteopedia
(Difference between revisions)
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<StructureSection load='6w9q' size='340' side='right'caption='[[6w9q]], [[Resolution|resolution]] 2.05Å' scene=''> | <StructureSection load='6w9q' size='340' side='right'caption='[[6w9q]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6w9q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[6w9q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W9Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W9Q FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w9q OCA], [http://pdbe.org/6w9q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w9q RCSB], [http://www.ebi.ac.uk/pdbsum/6w9q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w9q ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w9q OCA], [http://pdbe.org/6w9q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w9q RCSB], [http://www.ebi.ac.uk/pdbsum/6w9q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w9q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Many of the SARS-CoV-2 proteins have related counterparts across the Severe Acute Respiratory Syndrome (SARS-CoV) family. One such protein is non-structural protein 9 (Nsp9), which is thought to mediate viral replication, overall virulence, and viral genomic RNA reproduction. We sought to better characterize the SARS-CoV-2 Nsp9 and subsequently solved its X-ray crystal structure, in an apo form and, unexpectedly, in a peptide-bound form with a sequence originating from a rhinoviral 3C protease sequence (LEVL). The SARS-CoV-2 Nsp9 structure revealed the high level of structural conservation within the Nsp9 family. The exogenous peptide binding site is close to the dimer interface and impacted the relative juxtapositioning of the monomers within the homodimer. We have established a protocol for the production of SARS-CoV-2 Nsp9, determined its structure, and identified a peptide-binding site that warrants further study to understanding Nsp9 function. | ||
| + | |||
| + | Crystal Structure of the SARS-CoV-2 Non-structural Protein 9, Nsp9.,Littler DR, Gully BS, Colson RN, Rossjohn J iScience. 2020 Jul 24;23(7):101258. doi: 10.1016/j.isci.2020.101258. Epub 2020, Jun 9. PMID:32592996<ref>PMID:32592996</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6w9q" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: 2019-ncov]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Wcpv]] | ||
[[Category: Gully, B S]] | [[Category: Gully, B S]] | ||
[[Category: Littler, D R]] | [[Category: Littler, D R]] | ||
Revision as of 09:55, 9 September 2020
Peptide-bound SARS-CoV-2 Nsp9 RNA-replicase
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