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1b93

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{{STRUCTURE_1b93| PDB=1b93 | SCENE= }}
{{STRUCTURE_1b93| PDB=1b93 | SCENE= }}
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'''METHYLGLYOXAL SYNTHASE FROM ESCHERICHIA COLI'''
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===METHYLGLYOXAL SYNTHASE FROM ESCHERICHIA COLI===
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==Overview==
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BACKGROUND: The reaction mechanism of methylglyoxal synthase (MGS) is believed to be similar to that of triosephosphate isomerase (TIM). Both enzymes utilise dihydroxyacetone phosphate (DHAP) to form an enediol(ate) phosphate intermediate as the first step of their reaction pathways. However, the second catalytic step in the MGS reaction pathway is characterized by the elimination of phosphate and collapse of the enediol(ate) to form methylglyoxal instead of reprotonation to form the isomer glyceraldehyde 3-phosphate. RESULTS: The crystal structure of MGS bound to formate and substoichiometric amounts of phosphate in the space group P6522 has been determined at 1.9 A resolution. This structure shows that the enzyme is a homohexamer composed of interacting five-stranded beta/alpha proteins, rather than the hallmark alpha/beta barrel structure of TIM. The conserved residues His19, Asp71, and His98 in each of the three monomers in the asymmetric unit bind to a formate ion that is present in the crystallization conditions. Differences in the three monomers in the asymmetric unit are localized at the mouth of the active site and can be ascribed to the presence or absence of a bound phosphate ion. CONCLUSIONS: In agreement with site-directed mutagenesis and mechanistic enzymology, the structure suggests that Asp71 acts as the catalytic base. Further, Asp20 and Asp101 are involved in intersubunit salt bridges. These salt bridges may provide a pathway for transmitting allosteric information.
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(as it appears on PubMed at http://www.pubmed.gov), where 10368300 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10368300}}
==About this Structure==
==About this Structure==
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[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Methylglyoxal]]
[[Category: Methylglyoxal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:13:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:36:24 2008''

Revision as of 15:36, 30 June 2008

Image:1b93.png

Template:STRUCTURE 1b93

METHYLGLYOXAL SYNTHASE FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 10368300

About this Structure

1B93 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The crystal structure of methylglyoxal synthase from Escherichia coli., Saadat D, Harrison DH, Structure. 1999 Mar 15;7(3):309-17. PMID:10368300

Page seeded by OCA on Mon Jun 30 18:36:24 2008

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