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| | <StructureSection load='5gi0' size='340' side='right'caption='[[5gi0]], [[Resolution|resolution]] 2.04Å' scene=''> | | <StructureSection load='5gi0' size='340' side='right'caption='[[5gi0]], [[Resolution|resolution]] 2.04Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gi0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GI0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GI0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gi0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GI0 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.044Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MORF9, RIP9, At1g11430, T23J18.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gi0 OCA], [http://pdbe.org/5gi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gi0 RCSB], [http://www.ebi.ac.uk/pdbsum/5gi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gi0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gi0 OCA], [https://pdbe.org/5gi0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gi0 RCSB], [https://www.ebi.ac.uk/pdbsum/5gi0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gi0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MORF9_ARATH MORF9_ARATH]] Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).<ref>PMID:22411807</ref> <ref>PMID:23818871</ref> | + | [https://www.uniprot.org/uniprot/MORF9_ARATH MORF9_ARATH] Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).<ref>PMID:22411807</ref> <ref>PMID:23818871</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Guan, Z]] | + | [[Category: Guan Z]] |
| - | [[Category: Yan, J]] | + | [[Category: Yan J]] |
| - | [[Category: Yin, P]] | + | [[Category: Yin P]] |
| - | [[Category: Zhang, Q]] | + | [[Category: Zhang Q]] |
| - | [[Category: Zou, T]] | + | [[Category: Zou T]] |
| - | [[Category: Morf9]]
| + | |
| - | [[Category: Rip9]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna editing factor]]
| + | |
| Structural highlights
Function
MORF9_ARATH Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).[1] [2]
Publication Abstract from PubMed
RNA editing is a post-transcriptional process that modifies the genetic information on RNA molecules. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria. The PLS-type pentatricopeptide repeat (PPR) protein and the multiple organellar RNA editing factor (MORF, also known as RNA editing factor interacting protein (RIP)) are two types of key trans-acting factors involved in this process. However, how they cooperate with one another remains unclear. Here, we have characterized the interactions between a designer PLS-type PPR protein (PLS)3PPR and MORF9, and found that RNA-binding activity of (PLS)3PPR is drastically increased on MORF9 binding. We also determined the crystal structures of (PLS)3PPR, MORF9 and the (PLS)3PPR-MORF9 complex. MORF9 binding induces significant compressed conformational changes of (PLS)3PPR, revealing the molecular mechanisms by which MORF9-bound (PLS)3PPR has increased RNA-binding activity. Similarly, increased RNA-binding activity is observed for the natural PLS-type PPR protein, LPA66, in the presence of MORF9. These findings significantly expand our understanding of MORF function in plant organellar RNA editing.
MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing.,Yan J, Zhang Q, Guan Z, Wang Q, Li L, Ruan F, Lin R, Zou T, Yin P Nat Plants. 2017 Apr 10;3:17037. doi: 10.1038/nplants.2017.37. PMID:28394309[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Takenaka M, Zehrmann A, Verbitskiy D, Kugelmann M, Hartel B, Brennicke A. Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5104-9. doi:, 10.1073/pnas.1202452109. Epub 2012 Mar 12. PMID:22411807 doi:http://dx.doi.org/10.1073/pnas.1202452109
- ↑ Bentolila S, Oh J, Hanson MR, Bukowski R. Comprehensive high-resolution analysis of the role of an Arabidopsis gene family in RNA editing. PLoS Genet. 2013 Jun;9(6):e1003584. doi: 10.1371/journal.pgen.1003584. Epub 2013 , Jun 20. PMID:23818871 doi:http://dx.doi.org/10.1371/journal.pgen.1003584
- ↑ Yan J, Zhang Q, Guan Z, Wang Q, Li L, Ruan F, Lin R, Zou T, Yin P. MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing. Nat Plants. 2017 Apr 10;3:17037. doi: 10.1038/nplants.2017.37. PMID:28394309 doi:http://dx.doi.org/10.1038/nplants.2017.37
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