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| | ==Solution structure of the complex between DP1 acidic region and TFIIH p62 PH domain== | | ==Solution structure of the complex between DP1 acidic region and TFIIH p62 PH domain== |
| - | <StructureSection load='5gow' size='340' side='right'caption='[[5gow]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5gow' size='340' side='right'caption='[[5gow]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gow]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GOW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gow]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GOW FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTF2H1, BTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gow OCA], [http://pdbe.org/5gow PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gow RCSB], [http://www.ebi.ac.uk/pdbsum/5gow PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gow ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gow OCA], [https://pdbe.org/5gow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gow RCSB], [https://www.ebi.ac.uk/pdbsum/5gow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gow ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TF2H1_HUMAN TF2H1_HUMAN]] Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. | + | [https://www.uniprot.org/uniprot/TFDP1_HUMAN TFDP1_HUMAN] Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).<ref>PMID:20176812</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Nishimura, Y]] | + | [[Category: Nishimura Y]] |
| - | [[Category: Okuda, M]] | + | [[Category: Okuda M]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: General transcription factor]]
| + | |
| - | [[Category: Solution structure]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription activation]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| Structural highlights
Function
TFDP1_HUMAN Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).[1]
Publication Abstract from PubMed
The heterodimeric transcription factor E2F1-DP1 plays crucial roles in coordinating gene expression during G1/S cell cycle progression. For transcriptional activation, the transactivation domain (TAD) of E2F1 is known to interact with the TATA-binding protein of TFIID and the p62 subunit of TFIIH. It is generally believed that DP1 facilitates E2F1 binding to target DNA and does not possess a TAD. Here, we show that an acidic region of DP1, whose function has remained elusive, binds to the plekstrin homology (PH) domain of p62 with higher affinity than that of E2F1 and contributes to transcriptional activation. The structure of the complex revealed that DP1 forms a twisted U-shaped, string-like conformation and binds to the surface of the PH domain by anchoring Phe403 into a pocket in the PH domain. The transcriptional activity of E2F1-DP1 was reduced when Phe403 of DP1 was mutated. These findings indicate that the acidic region of DP1 acts as a TAD by contacting TFIIH.
The Interaction Mode of the Acidic Region of the Cell Cycle Transcription Factor DP1 with TFIIH.,Okuda M, Araki K, Ohtani K, Nishimura Y J Mol Biol. 2016 Dec 4;428(24 Pt B):4993-5006. doi: 10.1016/j.jmb.2016.11.001., Epub 2016 Nov 5. PMID:27825926[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zaragoza K, Begay V, Schuetz A, Heinemann U, Leutz A. Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes. Mol Cell Biol. 2010 May;30(9):2293-304. doi: 10.1128/MCB.01619-09. Epub 2010 Feb , 22. PMID:20176812 doi:http://dx.doi.org/10.1128/MCB.01619-09
- ↑ Okuda M, Araki K, Ohtani K, Nishimura Y. The Interaction Mode of the Acidic Region of the Cell Cycle Transcription Factor DP1 with TFIIH. J Mol Biol. 2016 Dec 4;428(24 Pt B):4993-5006. doi: 10.1016/j.jmb.2016.11.001., Epub 2016 Nov 5. PMID:27825926 doi:http://dx.doi.org/10.1016/j.jmb.2016.11.001
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