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| | <StructureSection load='5gpo' size='340' side='right'caption='[[5gpo]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='5gpo' size='340' side='right'caption='[[5gpo]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GPO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GPO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.701Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [http://pdbe.org/5gpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [http://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [https://pdbe.org/5gpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [https://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9I0V9_PSEAE Q9I0V9_PSEAE] Member of a two-component regulatory system.[RuleBase:RU364088] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Histidine kinase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, H]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Chen, W Z]] | + | [[Category: Chen H]] |
| - | [[Category: Gan, J H]] | + | [[Category: Chen WZ]] |
| - | [[Category: Hu, Q Y]] | + | [[Category: Gan JH]] |
| - | [[Category: Huang, S Q]] | + | [[Category: Hu QY]] |
| - | [[Category: Liu, X C]] | + | [[Category: Huang SQ]] |
| - | [[Category: Wang, D]] | + | [[Category: Liu XC]] |
| - | [[Category: Wei, T B]] | + | [[Category: Wang D]] |
| - | [[Category: Heavy metal]]
| + | [[Category: Wei TB]] |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Two-component system]]
| + | |
| Structural highlights
Function
Q9I0V9_PSEAE Member of a two-component regulatory system.[RuleBase:RU364088]
Publication Abstract from PubMed
Pseudomonas aeruginosa (P. aeruginosa) is a major opportunistic human pathogen, causing serious nosocomial infections among immunocompromised patients by multi-determinant virulence and high antibiotic resistance. The CzcR-CzcS signal transduction system in P. aeruginosa is primarily involved in metal detoxification and antibiotic resistance through co-regulating cross-resistance between Zn(II) and carbapenem antibiotics. Although the intracellular regulatory pathway is well-established, the mechanism by which extracellular sensor domain of histidine kinase (HK) CzcS responds to Zn(II) stimulus to trigger downstream signal transduction remains unclear. Here we determined the crystal structure of the CzcS sensor domain (CzcS SD) in complex with Zn(II) at 1.7 A resolution. This is the first three-dimensional structural view of Zn(II)-sensor domain of the two-component system (TCS). The CzcS SD is of alpha/beta-fold in nature, and it senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues (His55 and Asp60) on the dimer interface. Though the CzcS SD resembles the PhoQ-DcuS-CitA (PDC) superfamily member, it interacts with the effector in a novel domain with the N-terminal alpha-helices rather than the conserved beta-sheets pocket. The dimerization of the N-terminal H1 and H1' alpha-helices is of primary importance for the activity of HK CzcS. This study provides preliminary insight into the molecular mechanism of Zn(II) sensing and signaling transduction by the HK CzcS, which will be beneficial to understand how the pathogen P. aeruginosa resists to high levels of heavy metals and antimicrobial agents.
Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa.,Wang D, Chen W, Huang S, He Y, Liu X, Hu Q, Wei T, Sang H, Gan J, Chen H PLoS Pathog. 2017 Jul 21;13(7):e1006533. doi: 10.1371/journal.ppat.1006533., eCollection 2017 Jul. PMID:28732057[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang D, Chen W, Huang S, He Y, Liu X, Hu Q, Wei T, Sang H, Gan J, Chen H. Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa. PLoS Pathog. 2017 Jul 21;13(7):e1006533. doi: 10.1371/journal.ppat.1006533., eCollection 2017 Jul. PMID:28732057 doi:http://dx.doi.org/10.1371/journal.ppat.1006533
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