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| | <StructureSection load='5h7z' size='340' side='right'caption='[[5h7z]], [[Resolution|resolution]] 3.06Å' scene=''> | | <StructureSection load='5h7z' size='340' side='right'caption='[[5h7z]], [[Resolution|resolution]] 3.06Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5h7z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H7Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H7Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5h7z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H7Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.057Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h7y|5h7y]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h7z OCA], [https://pdbe.org/5h7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h7z RCSB], [https://www.ebi.ac.uk/pdbsum/5h7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h7z ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h7z OCA], [http://pdbe.org/5h7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h7z RCSB], [http://www.ebi.ac.uk/pdbsum/5h7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h7z ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9I3K3_PSEAE Q9I3K3_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cui, S]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Gao, X P]] | + | [[Category: Cui S]] |
| - | [[Category: Mu, Z X]] | + | [[Category: Gao XP]] |
| - | [[Category: Antimicrobial peptide]] | + | [[Category: Mu ZX]] |
| - | [[Category: Effector -immunity complex]]
| + | |
| - | [[Category: Hydrolase inhibitor]]
| + | |
| - | [[Category: Inhibitor]]
| + | |
| Structural highlights
Function
Q9I3K3_PSEAE
Publication Abstract from PubMed
The type VI secretion system (T6SS) secretes numerous toxins for bacteria-bacteria competition. TplE is a newly identified trans-kingdom toxin secreted by the T6SS in Pseudomonas aeruginosa, while TplEi neutralizes the toxic effect of TplE to protect bacteria autointoxication. Blocking the interaction of TplE-TplEi could unleash the toxin, causing bacterial cell death. In this study, we applied a crystallographic approach to design a structural-based antimicrobial peptides targeting the interaction of TplE and TplEi. We found that a peptide (designed as "L" peptide based on its shape) derived from TplE can form a crystal complex with TplEi after subtilisin treatment and the crystal structure was solved at 2.2A. The "L" peptide displays strong binding affinity to TplEi in vitro and can release the TplE toxin to induce bacteria death in vivo. Our findings suggest that as a toxin activator, the "L" peptide could be a possible drug lead for treating P. aeruginosa infection. Our findings provide an example that the T6SS effector and immunity protein could be a potential drug target against bacteria infection.
Structure-Based Prototype Peptides Targeting the Pseudomonas aeruginosa Type VI Secretion System Effector as a Novel Antibacterial Strategy.,Gao X, Mu Z, Qin B, Sun Y, Cui S Front Cell Infect Microbiol. 2017 Sep 20;7:411. doi: 10.3389/fcimb.2017.00411., eCollection 2017. PMID:28979890[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gao X, Mu Z, Qin B, Sun Y, Cui S. Structure-Based Prototype Peptides Targeting the Pseudomonas aeruginosa Type VI Secretion System Effector as a Novel Antibacterial Strategy. Front Cell Infect Microbiol. 2017 Sep 20;7:411. doi: 10.3389/fcimb.2017.00411., eCollection 2017. PMID:28979890 doi:http://dx.doi.org/10.3389/fcimb.2017.00411
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