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| | <StructureSection load='5hd2' size='340' side='right'caption='[[5hd2]], [[Resolution|resolution]] 2.28Å' scene=''> | | <StructureSection load='5hd2' size='340' side='right'caption='[[5hd2]], [[Resolution|resolution]] 2.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hd2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HD2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HD2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hd2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HD2 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.276Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hd2 OCA], [http://pdbe.org/5hd2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hd2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hd2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hd2 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hd2 OCA], [https://pdbe.org/5hd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hd2 RCSB], [https://www.ebi.ac.uk/pdbsum/5hd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hd2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/E9KBU4_BACTU E9KBU4_BACTU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus cereus var. thuringiensis smith et al. 1952]] | + | [[Category: Bacillus thuringiensis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Moshiri, F]] | + | [[Category: Moshiri F]] |
| - | [[Category: Rydel, T J]] | + | [[Category: Rydel TJ]] |
| - | [[Category: Sturman, E J]] | + | [[Category: Sturman EJ]] |
| - | [[Category: Bt]]
| + | |
| - | [[Category: Cry]]
| + | |
| - | [[Category: Cry51aa2]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
E9KBU4_BACTU
Publication Abstract from PubMed
Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.
A transgenic approach for controlling Lygus in cotton.,Gowda A, Rydel TJ, Wollacott AM, Brown RS, Akbar W, Clark TL, Flasinski S, Nageotte JR, Read AC, Shi X, Werner BJ, Pleau MJ, Baum JA Nat Commun. 2016 Jul 18;7:12213. doi: 10.1038/ncomms12213. PMID:27426014[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gowda A, Rydel TJ, Wollacott AM, Brown RS, Akbar W, Clark TL, Flasinski S, Nageotte JR, Read AC, Shi X, Werner BJ, Pleau MJ, Baum JA. A transgenic approach for controlling Lygus in cotton. Nat Commun. 2016 Jul 18;7:12213. doi: 10.1038/ncomms12213. PMID:27426014 doi:http://dx.doi.org/10.1038/ncomms12213
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