1be6

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{{STRUCTURE_1be6| PDB=1be6 | SCENE= }}
{{STRUCTURE_1be6| PDB=1be6 | SCENE= }}
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'''TRANS-CINNAMOYL-SUBTILISIN IN ANHYDROUS ACETONITRILE'''
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===TRANS-CINNAMOYL-SUBTILISIN IN ANHYDROUS ACETONITRILE===
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==Overview==
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The x-ray crystal structures of trans-cinnamoyl-subtilisin, an acyl-enzyme covalent intermediate of the serine protease subtilisin Carlsberg, have been determined to 2.2-A resolution in anhydrous acetonitrile and in water. The cinnamoyl-subtilisin structures are virtually identical in the two solvents. In addition, their enzyme portions are nearly indistinguishable from previously determined structures of the free enzyme in acetonitrile and in water; thus, acylation in either aqueous or nonaqueous solvent causes no appreciable conformational changes. However, the locations of bound solvent molecules in the active site of the acyl- and free enzyme forms in acetonitrile and in water are distinct. Such differences in the active site solvation may contribute to the observed variations in enzymatic activities. On prolonged exposure to organic solvent or removal of interstitial solvent from the crystal lattice, the channels within enzyme crystals are shown to collapse, leading to a drop in the number of active sites accessible to the substrate. The mechanistic and preparative implications of our findings for enzymatic catalysis in organic solvents are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 9789015 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9789015}}
==About this Structure==
==About this Structure==
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[[Category: Organic solvent]]
[[Category: Organic solvent]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:57:07 2008''

Revision as of 15:57, 30 June 2008

Image:1be6.png

Template:STRUCTURE 1be6

TRANS-CINNAMOYL-SUBTILISIN IN ANHYDROUS ACETONITRILE

Template:ABSTRACT PUBMED 9789015

About this Structure

1BE6 is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.

Reference

Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water., Schmitke JL, Stern LJ, Klibanov AM, Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12918-23. PMID:9789015

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