|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6sz9' size='340' side='right'caption='[[6sz9]], [[Resolution|resolution]] 3.70Å' scene=''> | | <StructureSection load='6sz9' size='340' side='right'caption='[[6sz9]], [[Resolution|resolution]] 3.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sz9]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SZ9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sz9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SZ9 FirstGlance]. <br> |
| | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">icmO, lpg0446 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152]), icmP, lpg0445 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152]), icmJ, lpg0455 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152]), lpg1549 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152]), lpg0294 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sz9 OCA], [https://pdbe.org/6sz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sz9 RCSB], [https://www.ebi.ac.uk/pdbsum/6sz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sz9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sz9 OCA], [http://pdbe.org/6sz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sz9 RCSB], [http://www.ebi.ac.uk/pdbsum/6sz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sz9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DOTL_LEGPH DOTL_LEGPH] Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). Plays a central role in the assembly of the subcomplex (PubMed:32513920). Required for the recruitment of IcmS and IcmW to the inner membrane and for the translocation of adapter-dependent substrates (PubMed:23028312). May have ATPase activity (Probable).<ref>PMID:17040490</ref> <ref>PMID:22694730</ref> <ref>PMID:23028312</ref> <ref>PMID:32513920</ref> <ref>PMID:32513920</ref> |
| | + | <div style="background-color:#fffaf0;"> |
| | + | == Publication Abstract from PubMed == |
| | + | Legionella pneumophila is a bacterial pathogen that utilises a Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential to the recruitment and delivery of effectors to the T4S machinery is the membrane-embedded T4 coupling complex (T4CC). Here, we purify an intact T4CC from the Legionella membrane. It contains the DotL ATPase, the DotM and DotN proteins, the chaperone module IcmSW, and two previously uncharacterised proteins, DotY and DotZ. The atomic resolution structure reveals a DotLMNYZ hetero-pentameric core from which the flexible IcmSW module protrudes. Six of these hetero-pentameric complexes may assemble into a 1.6-MDa hexameric nanomachine, forming an inner membrane channel for effectors to pass through. Analysis of multiple cryo EM maps, further modelling and mutagenesis provide working models for the mechanism for binding and delivery of two essential classes of Legionella effectors, depending on IcmSW or DotM, respectively. |
| | + | |
| | + | Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila.,Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920<ref>PMID:32513920</ref> |
| | + | |
| | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | + | </div> |
| | + | <div class="pdbe-citations 6sz9" style="background-color:#fffaf0;"></div> |
| | + | == References == |
| | + | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33152]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lukoyanova, N]] | + | [[Category: Legionella pneumophila]] |
| - | [[Category: Mace, K]] | + | [[Category: Lukoyanova N]] |
| - | [[Category: Meir, A]] | + | [[Category: Mace K]] |
| - | [[Category: Waksman, G]] | + | [[Category: Meir A]] |
| - | [[Category: Coupling protein]]
| + | [[Category: Waksman G]] |
| - | [[Category: Dotl]]
| + | |
| - | [[Category: Dotm]]
| + | |
| - | [[Category: Dotn]]
| + | |
| - | [[Category: Doty]]
| + | |
| - | [[Category: Dotz]]
| + | |
| - | [[Category: Gram-negative bacteria]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Secretion]]
| + | |
| - | [[Category: Secretion system]]
| + | |
| - | [[Category: T4cc]]
| + | |
| - | [[Category: T4ss]]
| + | |
| - | [[Category: Type 4 secretion system]]
| + | |
| Structural highlights
Function
DOTL_LEGPH Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). Plays a central role in the assembly of the subcomplex (PubMed:32513920). Required for the recruitment of IcmS and IcmW to the inner membrane and for the translocation of adapter-dependent substrates (PubMed:23028312). May have ATPase activity (Probable).[1] [2] [3] [4] [5]
Publication Abstract from PubMed
Legionella pneumophila is a bacterial pathogen that utilises a Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential to the recruitment and delivery of effectors to the T4S machinery is the membrane-embedded T4 coupling complex (T4CC). Here, we purify an intact T4CC from the Legionella membrane. It contains the DotL ATPase, the DotM and DotN proteins, the chaperone module IcmSW, and two previously uncharacterised proteins, DotY and DotZ. The atomic resolution structure reveals a DotLMNYZ hetero-pentameric core from which the flexible IcmSW module protrudes. Six of these hetero-pentameric complexes may assemble into a 1.6-MDa hexameric nanomachine, forming an inner membrane channel for effectors to pass through. Analysis of multiple cryo EM maps, further modelling and mutagenesis provide working models for the mechanism for binding and delivery of two essential classes of Legionella effectors, depending on IcmSW or DotM, respectively.
Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila.,Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vincent CD, Friedman JR, Jeong KC, Buford EC, Miller JL, Vogel JP. Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol. 2006 Dec;62(5):1278-91. doi: 10.1111/j.1365-2958.2006.05446.x. , Epub 2006 Oct 16. PMID:17040490 doi:http://dx.doi.org/10.1111/j.1365-2958.2006.05446.x
- ↑ Vincent CD, Friedman JR, Jeong KC, Sutherland MC, Vogel JP. Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol. 2012 Jul;85(2):378-91. doi: 10.1111/j.1365-2958.2012.08118.x. Epub , 2012 Jun 14. PMID:22694730 doi:http://dx.doi.org/10.1111/j.1365-2958.2012.08118.x
- ↑ Sutherland MC, Nguyen TL, Tseng V, Vogel JP. The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates. PLoS Pathog. 2012 Sep;8(9):e1002910. doi: 10.1371/journal.ppat.1002910. Epub 2012 , Sep 13. PMID:23028312 doi:http://dx.doi.org/10.1371/journal.ppat.1002910
- ↑ Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G. Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila. Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920 doi:http://dx.doi.org/10.1038/s41467-020-16681-z
- ↑ Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G. Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila. Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920 doi:http://dx.doi.org/10.1038/s41467-020-16681-z
- ↑ Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G. Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila. Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920 doi:http://dx.doi.org/10.1038/s41467-020-16681-z
|
