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| | <StructureSection load='5hnp' size='340' side='right'caption='[[5hnp]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5hnp' size='340' side='right'caption='[[5hnp]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hnp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_31898 Atcc 31898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HNP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HNP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hnp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Raoultella_ornithinolytica Raoultella ornithinolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HNP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hno|5hno]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TE10_19180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54291 ATCC 31898])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hnp OCA], [https://pdbe.org/5hnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hnp RCSB], [https://www.ebi.ac.uk/pdbsum/5hnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hnp ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hnp OCA], [http://pdbe.org/5hnp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hnp RCSB], [http://www.ebi.ac.uk/pdbsum/5hnp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hnp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 31898]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kimber, M S]] | + | [[Category: Raoultella ornithinolytica]] |
| - | [[Category: Mallette, E]] | + | [[Category: Kimber MS]] |
| - | [[Category: Mann, E]] | + | [[Category: Mallette E]] |
| - | [[Category: Whitfield, C]] | + | [[Category: Mann E]] |
| - | [[Category: Abc transporter]] | + | [[Category: Whitfield C]] |
| - | [[Category: Carbohydrate binding subunit]]
| + | |
| - | [[Category: O antigen export]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Export of the Escherichia coli serotype O9a O-antigenic polysaccharides (O-PS) involves an ATP-binding cassette (ABC) transporter. The process requires a non-reducing terminal residue, which is recognized by a carbohydrate-binding module (CBM) appended to the C terminus of the nucleotide-binding domain of the transporter. Here, we investigate the process in Klebsiella pneumoniae serotype O12 (and Raoultella terrigena ATCC 33257). The O12 polysaccharide is terminated at the non-reducing end by a beta-linked 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) residue. The O12 ABC transporter also binds its cognate O-PS via a CBM, and export is dependent on the presence of the terminal beta-Kdo residue. The overall structural architecture of the O12 CBM resembles the O9a prototype, but they share only weak sequence similarity, and the putative binding pocket for the O12 glycan is different. Removal of the CBM abrogated O-PS transport, but export was restored when the CBM was expressed in trans with the mutant CBM-deficient ABC transporter. These results demonstrate that the CBM-mediated substrate-recognition mechanism is evolutionarily conserved and can operate with glycans of widely differing structures.
The Klebsiella pneumoniae O12 ATP-binding Cassette (ABC) Transporter Recognizes the Terminal Residue of Its O-antigen Polysaccharide Substrate.,Mann E, Mallette E, Clarke BR, Kimber MS, Whitfield C J Biol Chem. 2016 Apr 29;291(18):9748-61. doi: 10.1074/jbc.M116.719344. Epub 2016, Mar 2. PMID:26934919[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mann E, Mallette E, Clarke BR, Kimber MS, Whitfield C. The Klebsiella pneumoniae O12 ATP-binding Cassette (ABC) Transporter Recognizes the Terminal Residue of Its O-antigen Polysaccharide Substrate. J Biol Chem. 2016 Apr 29;291(18):9748-61. doi: 10.1074/jbc.M116.719344. Epub 2016, Mar 2. PMID:26934919 doi:http://dx.doi.org/10.1074/jbc.M116.719344
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