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| | <StructureSection load='5hwo' size='340' side='right'caption='[[5hwo]], [[Resolution|resolution]] 1.48Å' scene=''> | | <StructureSection load='5hwo' size='340' side='right'caption='[[5hwo]], [[Resolution|resolution]] 1.48Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hwo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HWO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hwo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HWO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HMG:3-HYDROXY-3-METHYLGLUTARYL-COENZYME+A'>HMG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mvaS, MXAN_4267 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246197 MYXXD])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HMG:3-HYDROXY-3-METHYLGLUTARYL-COENZYME+A'>HMG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydroxymethylglutaryl-CoA_synthase Hydroxymethylglutaryl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.10 2.3.3.10] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwo OCA], [https://pdbe.org/5hwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hwo RCSB], [https://www.ebi.ac.uk/pdbsum/5hwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwo ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwo OCA], [http://pdbe.org/5hwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hwo RCSB], [http://www.ebi.ac.uk/pdbsum/5hwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q1D4I1_MYXXD Q1D4I1_MYXXD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hydroxymethylglutaryl-CoA synthase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myxxd]] | + | [[Category: Myxococcus xanthus DK 1622]] |
| - | [[Category: Blankenfeldt, W]] | + | [[Category: Blankenfeldt W]] |
| - | [[Category: Bock, T]] | + | [[Category: Bock T]] |
| - | [[Category: Kasten, J]] | + | [[Category: Kasten J]] |
| - | [[Category: Biosynthesis]]
| + | |
| - | [[Category: Mva]]
| + | |
| - | [[Category: Myxococcus xanthus]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q1D4I1_MYXXD
Publication Abstract from PubMed
A critical step in bacterial isoprenoid production is the synthesis of 3-hydroxy-3-methylglutaryl coenzyme A catalyzed by HMG-CoA synthase (HMGCS). In myxobacteria, this enzyme is also involved in a recently discovered acetyl-CoA-dependent isovaleryl-CoA biosynthesis pathway. Here we present crystal structures of MvaS, the HMGCS from Myxococcus xanthus, in complex with coenzyme A and acetylated active site Cys115, with the second substrate acetoacetyl-CoA and with the product 3-hydroxy-3-methylglutaryl-CoA. We show that MvaS uses the common HMGCS enzymatic mechanism and provide evidence that dimerization plays a role in the formation and stability of the active site. Overall, MvaS shows typical features of the eukaryotic HMGCS and exhibits differences to homologs from Gram-positive bacteria. This study provides insights into myxobacterial alternative isovaleryl coenzyme A biosynthesis and thereby extends the toolbox for the biotechnological production of renewable fuel and chemicals.
Crystal structure of the HMG-CoA synthase MvaS from the Gram-negative bacterium Myxococcus xanthus.,Bock T, Kasten J, Muller R, Blankenfeldt W Chembiochem. 2016 Apr 28. doi: 10.1002/cbic.201600070. PMID:27124816[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bock T, Kasten J, Muller R, Blankenfeldt W. Crystal structure of the HMG-CoA synthase MvaS from the Gram-negative bacterium Myxococcus xanthus. Chembiochem. 2016 Apr 28. doi: 10.1002/cbic.201600070. PMID:27124816 doi:http://dx.doi.org/10.1002/cbic.201600070
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