1bf4

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{{STRUCTURE_1bf4| PDB=1bf4 | SCENE= }}
{{STRUCTURE_1bf4| PDB=1bf4 | SCENE= }}
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'''CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX'''
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===CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX===
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==Overview==
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Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing.
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{{ABSTRACT_PUBMED_9731772}}
==About this Structure==
==About this Structure==
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[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
[[Category: Hyperthermophile]]
[[Category: Hyperthermophile]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:25:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:00:56 2008''

Revision as of 16:00, 30 June 2008

Image:1bf4.png

Template:STRUCTURE 1bf4

CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX

Template:ABSTRACT PUBMED 9731772

About this Structure

1BF4 is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA.

Reference

The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA., Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary BS, Edmondson SP, Shriver JW, Wang AH, Nat Struct Biol. 1998 Sep;5(9):782-6. PMID:9731772

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