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6ww7

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Structure of the human ER membrane protein complex in a lipid nanodisc

Structural highlights

6ww7 is a 9 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[EMC1_HUMAN] Global developmental delay-visual anomalies-progressive cerebellar atrophy-truncal hypotonia syndrome. The disease is caused by mutations affecting the gene represented in this entry.

Function

[EMC10_HUMAN] Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways.^[1] [MMGT1_HUMAN] Mediates Mg(2+) transport.[UniProtKB:Q8K273]

Publication Abstract from PubMed

A defining step in the biogenesis of a membrane protein is the insertion of its hydrophobic transmembrane helices into the lipid bilayer. The nine-subunit ER membrane protein complex (EMC) is a conserved co- and post-translational insertase at the endoplasmic reticulum. We determined the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4 A by cryo-electron microscopy, permitting building of a nearly complete atomic model. We used structure-guided mutagenesis to demonstrate that substrate insertion requires a methionine-rich cytosolic loop and occurs via an enclosed hydrophilic vestibule within the membrane formed by the subunits EMC3 and EMC6. We propose that the EMC uses local membrane thinning and a positively charged patch to decrease the energetic barrier for insertion into the bilayer.

Structural basis for membrane insertion by the human ER membrane protein complex.,Pleiner T, Pinton Tomaleri G, Januszyk K, Inglis AJ, Hazu M, Voorhees RM Science. 2020 May 21. pii: science.abb5008. doi: 10.1126/science.abb5008. PMID:32439656^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reboll MR, Korf-Klingebiel M, Klede S, Polten F, Brinkmann E, Reimann I, Schonfeld HJ, Bobadilla M, Faix J, Kensah G, Gruh I, Klintschar M, Gaestel M, Niessen HW, Pich A, Bauersachs J, Gogos JA, Wang Y, Wollert KC. EMC10 (Endoplasmic Reticulum Membrane Protein Complex Subunit 10) Is a Bone Marrow-Derived Angiogenic Growth Factor Promoting Tissue Repair After Myocardial Infarction. Circulation. 2017 Nov 7;136(19):1809-1823. doi:, 10.1161/CIRCULATIONAHA.117.029980. Epub 2017 Sep 20. PMID:28931551 doi:http://dx.doi.org/10.1161/CIRCULATIONAHA.117.029980
↑ Pleiner T, Pinton Tomaleri G, Januszyk K, Inglis AJ, Hazu M, Voorhees RM. Structural basis for membrane insertion by the human ER membrane protein complex. Science. 2020 May 21. pii: science.abb5008. doi: 10.1126/science.abb5008. PMID:32439656 doi:http://dx.doi.org/10.1126/science.abb5008

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ww7"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ww7' size='340' side='right'caption='[[6ww7]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ww7]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WW7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WW7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ww7]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WW7 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ww7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ww7 OCA], [http://pdbe.org/6ww7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ww7 RCSB], [http://www.ebi.ac.uk/pdbsum/6ww7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ww7 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ww7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ww7 OCA], [https://pdbe.org/6ww7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ww7 RCSB], [https://www.ebi.ac.uk/pdbsum/6ww7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ww7 ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/EMC1_HUMAN EMC1_HUMAN]] Global developmental delay-visual anomalies-progressive cerebellar atrophy-truncal hypotonia syndrome. The disease is caused by mutations affecting the gene represented in this entry.
+[[https://www.uniprot.org/uniprot/EMC1_HUMAN EMC1_HUMAN]] Global developmental delay-visual anomalies-progressive cerebellar atrophy-truncal hypotonia syndrome. The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/EMC10_HUMAN EMC10_HUMAN]] Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways.<ref>PMID:28931551</ref>  [[http://www.uniprot.org/uniprot/MMGT1_HUMAN MMGT1_HUMAN]] Mediates Mg(2+) transport.[UniProtKB:Q8K273]
+[[https://www.uniprot.org/uniprot/EMC10_HUMAN EMC10_HUMAN]] Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways.<ref>PMID:28931551</ref>  [[https://www.uniprot.org/uniprot/MMGT1_HUMAN MMGT1_HUMAN]] Mediates Mg(2+) transport.[UniProtKB:Q8K273]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

6ww7

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Current revision

Structure of the human ER membrane protein complex in a lipid nanodisc

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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