1a9m
From Proteopedia
(New page: 200px<br /> <applet load="1a9m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a9m, resolution 2.3Å" /> '''G48H MUTANT OF HIV-1...) |
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| - | [[Image:1a9m.gif|left|200px]]<br /> | + | [[Image:1a9m.gif|left|200px]]<br /><applet load="1a9m" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1a9m" size=" | + | |
caption="1a9m, resolution 2.3Å" /> | caption="1a9m, resolution 2.3Å" /> | ||
'''G48H MUTANT OF HIV-1 PROTEASE IN COMPLEX WITH A PEPTIDIC INHIBITOR U-89360E'''<br /> | '''G48H MUTANT OF HIV-1 PROTEASE IN COMPLEX WITH A PEPTIDIC INHIBITOR U-89360E'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of human immunodeficiency virus type 1 (HIV-1) | + | The crystal structure of human immunodeficiency virus type 1 (HIV-1) protease mutant G48H with peptidic inhibitor U-89360E is described. Comparison with wild-type protease-inhibitor complex shows that mutation of flap residue 48 to histidine allows stabilizing van der Waals contacts between the side chains of His48 and Phe53 as well as between His48 and the P2' and P3' inhibitor subsites. The flap region is less mobile than in the wild-type enzyme. A model of saquinavir-resistant mutant protease G48V in complex with saquinavir predicts interactions similar to those found in the G48H crystal. Energetic calculations confirm the similarity of the His48 and Val48 interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1A9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] with U0E as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1A9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] with <scene name='pdbligand=U0E:'>U0E</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Foundling, S.]] | [[Category: Foundling, S.]] | ||
| - | [[Category: Hartsuck, J | + | [[Category: Hartsuck, J A.]] |
[[Category: Hong, L.]] | [[Category: Hong, L.]] | ||
[[Category: Tang, J.]] | [[Category: Tang, J.]] | ||
| - | [[Category: Zhang, X | + | [[Category: Zhang, X J.]] |
[[Category: U0E]] | [[Category: U0E]] | ||
[[Category: aspartyl protease]] | [[Category: aspartyl protease]] | ||
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[[Category: mutation]] | [[Category: mutation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:20 2008'' |
Revision as of 09:42, 21 February 2008
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G48H MUTANT OF HIV-1 PROTEASE IN COMPLEX WITH A PEPTIDIC INHIBITOR U-89360E
Overview
The crystal structure of human immunodeficiency virus type 1 (HIV-1) protease mutant G48H with peptidic inhibitor U-89360E is described. Comparison with wild-type protease-inhibitor complex shows that mutation of flap residue 48 to histidine allows stabilizing van der Waals contacts between the side chains of His48 and Phe53 as well as between His48 and the P2' and P3' inhibitor subsites. The flap region is less mobile than in the wild-type enzyme. A model of saquinavir-resistant mutant protease G48V in complex with saquinavir predicts interactions similar to those found in the G48H crystal. Energetic calculations confirm the similarity of the His48 and Val48 interactions.
About this Structure
1A9M is a Single protein structure of sequence from Human immunodeficiency virus with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a G48H mutant of HIV-1 protease explains how glycine-48 replacements produce mutants resistant to inhibitor drugs., Hong L, Zhang XJ, Foundling S, Hartsuck JA, Tang J, FEBS Lett. 1997 Dec 22;420(1):11-6. PMID:9450540
Page seeded by OCA on Thu Feb 21 11:42:20 2008
