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| | <StructureSection load='5j9i' size='340' side='right'caption='[[5j9i]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='5j9i' size='340' side='right'caption='[[5j9i]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5j9i]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J9I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5j9i]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J9I FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">higA-2, VC_A0469 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.797Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9i OCA], [http://pdbe.org/5j9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j9i RCSB], [http://www.ebi.ac.uk/pdbsum/5j9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9i ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j9i OCA], [https://pdbe.org/5j9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j9i RCSB], [https://www.ebi.ac.uk/pdbsum/5j9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j9i ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HIGA2_VIBCH HIGA2_VIBCH]] Antitoxin component of a toxin-antitoxin (TA) module that counteracts the effect of the HigB-2 toxin. Binds to its own promoter and regulates transcription of the higB-2/higA-2 operon.<ref>PMID:17020579</ref> | + | [https://www.uniprot.org/uniprot/HIGA2_VIBCH HIGA2_VIBCH] Antitoxin component of a toxin-antitoxin (TA) module that counteracts the effect of the HigB-2 toxin. Binds to its own promoter and regulates transcription of the higB-2/higA-2 operon.<ref>PMID:17020579</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hadzi, S]] | + | [[Category: Vibrio cholerae]] |
| - | [[Category: Loris, R]] | + | [[Category: Hadzi S]] |
| - | [[Category: Antitoxin]] | + | [[Category: Loris R]] |
| - | [[Category: Toxin-antitoxin system]]
| + | |
| Structural highlights
Function
HIGA2_VIBCH Antitoxin component of a toxin-antitoxin (TA) module that counteracts the effect of the HigB-2 toxin. Binds to its own promoter and regulates transcription of the higB-2/higA-2 operon.[1]
Publication Abstract from PubMed
Toxin-antitoxin (TA) modules are small operons involved in bacterial stress response and persistence. higBA operons form a family of TA modules with an inverted gene organization and a toxin belonging to the RelE/ParE superfamily. Here, we present the crystal structures of chromosomally encoded Vibrio cholerae antitoxin (VcHigA2), toxin (VcHigB2) and their complex, which show significant differences in structure and mechanisms of function compared to the higBA module from plasmid Rts1, the defining member of the family. The VcHigB2 is more closely related to Escherichia coli RelE both in terms of overall structure and the organization of its active site. VcHigB2 is neutralized by VcHigA2, a modular protein with an N-terminal intrinsically disordered toxin-neutralizing segment followed by a C-terminal helix-turn-helix dimerization and DNA binding domain. VcHigA2 binds VcHigB2 with picomolar affinity, which is mainly a consequence of entropically favorable de-solvation of a large hydrophobic binding interface and enthalpically favorable folding of the N-terminal domain into an alpha-helix followed by a beta-strand. This interaction displaces helix alpha3 of VcHigB2 and at the same time induces a one-residue shift in the register of beta-strand beta3, thereby flipping the catalytically important Arg64 out of the active site.
Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a beta-strand sliding mechanism.,Hadzi S, Garcia-Pino A, Haesaerts S, Jurenas D, Gerdes K, Lah J, Loris R Nucleic Acids Res. 2017 Feb 28. doi: 10.1093/nar/gkx138. PMID:28334932[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Christensen-Dalsgaard M, Gerdes K. Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving enzymes and can stabilize plasmids. Mol Microbiol. 2006 Oct;62(2):397-411. PMID:17020579 doi:http://dx.doi.org/10.1111/j.1365-2958.2006.05385.x
- ↑ Hadzi S, Garcia-Pino A, Haesaerts S, Jurenas D, Gerdes K, Lah J, Loris R. Ribosome-dependent Vibrio cholerae mRNAse HigB2 is regulated by a beta-strand sliding mechanism. Nucleic Acids Res. 2017 Feb 28. doi: 10.1093/nar/gkx138. PMID:28334932 doi:http://dx.doi.org/10.1093/nar/gkx138
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