1b03
From Proteopedia
(New page: 200px<br /> <applet load="1b03" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b03" /> '''SOLUTION STRUCTURE OF THE ANTIBODY-BOUND HI...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1b03.gif|left|200px]]<br /> | + | [[Image:1b03.gif|left|200px]]<br /><applet load="1b03" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1b03" size=" | + | |
caption="1b03" /> | caption="1b03" /> | ||
'''SOLUTION STRUCTURE OF THE ANTIBODY-BOUND HIV-1IIIB V3 PEPTIDE'''<br /> | '''SOLUTION STRUCTURE OF THE ANTIBODY-BOUND HIV-1IIIB V3 PEPTIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The refined solution structure of an 18-residue HIV-1IIIB V3 peptide in | + | The refined solution structure of an 18-residue HIV-1IIIB V3 peptide in complex with the Fv fragment of an anti-gp120 antibody reveals an unexpected type VI beta-turn comprising residues RGPG at the center of a beta-hairpin. The central glycine and proline of this turn are linked by a cis peptide bond. The residues of the turn interact extensively with the antibody Fv. 15N[1H] NOE measurements show that the backbone of the peptide, including the central QRGPGR loop, is well ordered in the complex. The solution structure is significantly different from the X-ray structures of HIV-1MN V3 peptides bound to anti-peptide antibodies. These differences could be due to a two-residue (QR) insertion preceding the GPGR sequence in the HIV-1IIIB strain, and the much longer peptide epitope immobilized by the anti-gp120 antibody. |
==About this Structure== | ==About this Structure== | ||
| - | 1B03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1B03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B03 OCA]. |
==Reference== | ==Reference== | ||
| Line 19: | Line 18: | ||
[[Category: p1053 structure]] | [[Category: p1053 structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:50:05 2008'' |
Revision as of 09:50, 21 February 2008
|
SOLUTION STRUCTURE OF THE ANTIBODY-BOUND HIV-1IIIB V3 PEPTIDE
Overview
The refined solution structure of an 18-residue HIV-1IIIB V3 peptide in complex with the Fv fragment of an anti-gp120 antibody reveals an unexpected type VI beta-turn comprising residues RGPG at the center of a beta-hairpin. The central glycine and proline of this turn are linked by a cis peptide bond. The residues of the turn interact extensively with the antibody Fv. 15N[1H] NOE measurements show that the backbone of the peptide, including the central QRGPGR loop, is well ordered in the complex. The solution structure is significantly different from the X-ray structures of HIV-1MN V3 peptides bound to anti-peptide antibodies. These differences could be due to a two-residue (QR) insertion preceding the GPGR sequence in the HIV-1IIIB strain, and the much longer peptide epitope immobilized by the anti-gp120 antibody.
About this Structure
1B03 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
A cis proline turn linking two beta-hairpin strands in the solution structure of an antibody-bound HIV-1IIIB V3 peptide., Tugarinov V, Zvi A, Levy R, Anglister J, Nat Struct Biol. 1999 Apr;6(4):331-5. PMID:10201400
Page seeded by OCA on Thu Feb 21 11:50:05 2008
