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| - | [[Image:1bj4.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1bj4| PDB=1bj4 | SCENE= }} | | {{STRUCTURE_1bj4| PDB=1bj4 | SCENE= }} |
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| - | '''RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)'''
| + | ===RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)=== |
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| - | ==Overview==
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| - | BACKGROUND: Serine hydroxymethyltransferase (SHMT) is a ubiquitous enzyme found in all prokaryotes and eukaryotes. As an enzyme of the thymidylate synthase metabolic cycle, SHMT catalyses the retro-aldol cleavage of serine to glycine, with the resulting hydroxymethyl group being transferred to tetrahydrofolate to form 5, 10-methylene-tetrahydrofolate. The latter is the major source of one-carbon units in metabolism. Elevated SHMT activity has been shown to be coupled to the increased demand for DNA synthesis in rapidly proliferating cells, particularly tumour cells. Consequently, the central role of SHMT in nucleotide biosynthesis makes it an attractive target for cancer chemotherapy. RESULTS: We have solved the crystal structure of human cytosolic SHMT by multiple isomorphous replacement to 2.65 A resolution. The monomer has a fold typical for alpha class pyridoxal 5'-phosphate (PLP) dependent enzymes. The tetramer association is best described as a 'dimer of dimers' where residues from both subunits of one 'tight' dimer contribute to the active site. CONCLUSIONS: The crystal structure shows the evolutionary relationship between SHMT and other alpha class PLP-dependent enzymes, as the fold is highly conserved. Many of the results of site-directed mutagenesis studies can easily be rationalised or re-interpreted in light of the structure presented here. For example, His 151 is not the catalytic base, contrary to the findings of others. A mechanism for the cleavage of serine to glycine and formaldehyde is proposed.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9753690}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9753690 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9753690}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Pyridoxal 5'-phosphate]] | | [[Category: Pyridoxal 5'-phosphate]] |
| | [[Category: Transferase]] | | [[Category: Transferase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:34:51 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:14:49 2008'' |
Revision as of 16:14, 30 June 2008
Template:STRUCTURE 1bj4
RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)
Template:ABSTRACT PUBMED 9753690
About this Structure
1BJ4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of human cytosolic serine hydroxymethyltransferase: a target for cancer chemotherapy., Renwick SB, Snell K, Baumann U, Structure. 1998 Sep 15;6(9):1105-16. PMID:9753690
Page seeded by OCA on Mon Jun 30 19:14:49 2008
