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| | <StructureSection load='6nke' size='340' side='right'caption='[[6nke]], [[Resolution|resolution]] 1.72Å' scene=''> | | <StructureSection load='6nke' size='340' side='right'caption='[[6nke]], [[Resolution|resolution]] 1.72Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nke]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thevo Thevo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NKE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NKE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nke]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_volcanium_GSS1 Thermoplasma volcanium GSS1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NKE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TV0745, TVG0750626 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273116 THEVO])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoglycerol_geranylgeranyltransferase Phosphoglycerol geranylgeranyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.41 2.5.1.41] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nke OCA], [https://pdbe.org/6nke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nke RCSB], [https://www.ebi.ac.uk/pdbsum/6nke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nke ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nke OCA], [http://pdbe.org/6nke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nke RCSB], [http://www.ebi.ac.uk/pdbsum/6nke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nke ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GGGPS_THEVO GGGPS_THEVO]] Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.[HAMAP-Rule:MF_00112] | + | [https://www.uniprot.org/uniprot/GGGPS_THEVO GGGPS_THEVO] Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.[HAMAP-Rule:MF_00112] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phosphoglycerol geranylgeranyltransferase]] | + | [[Category: Thermoplasma volcanium GSS1]] |
| - | [[Category: Thevo]]
| + | [[Category: Alderfer KE]] |
| - | [[Category: Alderfer, K E]] | + | [[Category: Blank PN]] |
| - | [[Category: Blank, P N]] | + | [[Category: Christianson DW]] |
| - | [[Category: Christianson, D W]] | + | [[Category: Gillott BN]] |
| - | [[Category: Gillott, B N]] | + | [[Category: Himmelberger JA]] |
| - | [[Category: Himmelberger, J A]] | + | |
| - | [[Category: Archaea complex tim-barrel transferase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
GGGPS_THEVO Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.[HAMAP-Rule:MF_00112]
Publication Abstract from PubMed
Archaea are uniquely adapted to thrive in harsh environments, and one of these adaptations involves the archaeal membrane lipids, which are characterized by their isoprenoid alkyl chains connected via ether linkages to glycerol 1-phosphate. The membrane lipids of the thermophilic and acidophilic euryarchaeota Thermoplasma volcanium are exclusively glycerol dibiphytanyl glycerol tetraethers. The first committed step in the biosynthetic pathway of these archaeal lipids is the formation of the ether linkage between glycerol 1-phosphate and geranylgeranyl diphosphate, and is catalyzed by the enzyme geranylgeranylglyceryl phosphate synthase (GGGPS). The 1.72 A resolution crystal structure of GGGPS from T. volcanium (TvGGGPS) in complex with glycerol and sulfate is reported here. The crystal structure reveals TvGGGPS to be a dimer, which is consistent with the absence of the aromatic anchor residue in helix alpha5a that is required for hexamerization in other GGGPS homologs; the hexameric quaternary structure in GGGPS is thought to provide thermostability. A phylogenetic analysis of the Euryarchaeota and a parallel ancestral state reconstruction investigated the relationship between optimal growth temperature and the ancestral sequences. The presence of an aromatic anchor residue is not explained by temperature as an ecological parameter. An examination of the active site of the TvGGGPS dimer revealed that it may be able to accommodate longer isoprenoid substrates, supporting an alternative pathway of isoprenoid membrane-lipid synthesis.
Structural studies of geranylgeranylglyceryl phosphate synthase, a prenyltransferase found in thermophilic Euryarchaeota.,Blank PN, Barnett AA, Ronnebaum TA, Alderfer KE, Gillott BN, Christianson DW, Himmelberger JA Acta Crystallogr D Struct Biol. 2020 Jun 1;76(Pt 6):542-557. doi:, 10.1107/S2059798320004878. Epub 2020 May 29. PMID:32496216[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Blank PN, Barnett AA, Ronnebaum TA, Alderfer KE, Gillott BN, Christianson DW, Himmelberger JA. Structural studies of geranylgeranylglyceryl phosphate synthase, a prenyltransferase found in thermophilic Euryarchaeota. Acta Crystallogr D Struct Biol. 2020 Jun 1;76(Pt 6):542-557. doi:, 10.1107/S2059798320004878. Epub 2020 May 29. PMID:32496216 doi:http://dx.doi.org/10.1107/S2059798320004878
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