1baj
From Proteopedia
(New page: 200px<br /> <applet load="1baj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1baj, resolution 2.60Å" /> '''HIV-1 CAPSID PROTEI...) |
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| - | [[Image:1baj.gif|left|200px]]<br /> | + | [[Image:1baj.gif|left|200px]]<br /><applet load="1baj" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1baj" size=" | + | |
caption="1baj, resolution 2.60Å" /> | caption="1baj, resolution 2.60Å" /> | ||
'''HIV-1 CAPSID PROTEIN C-TERMINAL FRAGMENT PLUS GAG P2 DOMAIN'''<br /> | '''HIV-1 CAPSID PROTEIN C-TERMINAL FRAGMENT PLUS GAG P2 DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human immunodeficiency virus type I (HIV-1) capsid protein is | + | The human immunodeficiency virus type I (HIV-1) capsid protein is initially synthesized as the central domain of the Gag polyprotein, and is subsequently proteolytically processed into a discrete 231-amino-acid protein that forms the distinctive conical core of the mature virus. The crystal structures of two proteins that span the C-terminal domain of the capsid are reported here: one encompassing residues 146-231 (CA146-231) and the other extending to include the 14-residue p2 domain of Gag (CA146-p2). The isomorphous CA146-231 and CA146-p2 structures were determined by molecular replacement and have been refined at 2.6 A resolution to R factors of 22.3 and 20.7% (Rfree = 28.1 and 27.5%), respectively. The ordered domains comprise residues 148-219 for CA146-231 and 148-218 for CA146-p2, and their refined structures are essentially identical. The proteins are composed of a 310 helix followed by an extended strand and four alpha-helices. A crystallographic twofold generates a dimer that is stabilized by parallel packing of an alpha-helix 2 across the dimer interface and by packing of the 310 helix into a groove created by alpha-helices 2 and 3 of the partner molecule. CA146-231 and CA146-p2 dimerize with the full affinity of the intact capsid protein, and their structures therefore reveal the essential dimer interface of the HIV-1 capsid. |
==About this Structure== | ==About this Structure== | ||
| - | 1BAJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http:// | + | 1BAJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Human immunodeficiency virus 1]] | [[Category: Human immunodeficiency virus 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hill, C | + | [[Category: Hill, C P.]] |
| - | [[Category: Sundquist, W | + | [[Category: Sundquist, W I.]] |
[[Category: Wang, H.]] | [[Category: Wang, H.]] | ||
| - | [[Category: Worthylake, D | + | [[Category: Worthylake, D K.]] |
[[Category: Yoo, S.]] | [[Category: Yoo, S.]] | ||
[[Category: capsid]] | [[Category: capsid]] | ||
[[Category: hiv-1 assembly protein]] | [[Category: hiv-1 assembly protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:53:13 2008'' |
Revision as of 09:53, 21 February 2008
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HIV-1 CAPSID PROTEIN C-TERMINAL FRAGMENT PLUS GAG P2 DOMAIN
Overview
The human immunodeficiency virus type I (HIV-1) capsid protein is initially synthesized as the central domain of the Gag polyprotein, and is subsequently proteolytically processed into a discrete 231-amino-acid protein that forms the distinctive conical core of the mature virus. The crystal structures of two proteins that span the C-terminal domain of the capsid are reported here: one encompassing residues 146-231 (CA146-231) and the other extending to include the 14-residue p2 domain of Gag (CA146-p2). The isomorphous CA146-231 and CA146-p2 structures were determined by molecular replacement and have been refined at 2.6 A resolution to R factors of 22.3 and 20.7% (Rfree = 28.1 and 27.5%), respectively. The ordered domains comprise residues 148-219 for CA146-231 and 148-218 for CA146-p2, and their refined structures are essentially identical. The proteins are composed of a 310 helix followed by an extended strand and four alpha-helices. A crystallographic twofold generates a dimer that is stabilized by parallel packing of an alpha-helix 2 across the dimer interface and by packing of the 310 helix into a groove created by alpha-helices 2 and 3 of the partner molecule. CA146-231 and CA146-p2 dimerize with the full affinity of the intact capsid protein, and their structures therefore reveal the essential dimer interface of the HIV-1 capsid.
About this Structure
1BAJ is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
Reference
Structures of the HIV-1 capsid protein dimerization domain at 2.6 A resolution., Worthylake DK, Wang H, Yoo S, Sundquist WI, Hill CP, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):85-92. Epub 1999, Jan 1. PMID:10089398
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