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1bjh

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{{STRUCTURE_1bjh| PDB=1bjh | SCENE= }}
{{STRUCTURE_1bjh| PDB=1bjh | SCENE= }}
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'''HAIRPIN LOOPS CONSISTING OF SINGLE ADENINE RESIDUES CLOSED BY SHEARED A(DOT)A AND G(DOT)G PAIRS FORMED BY THE DNA TRIPLETS AAA AND GAG: SOLUTION STRUCTURE OF THE D(GTACAAAGTAC) HAIRPIN, NMR, 16 STRUCTURES'''
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===HAIRPIN LOOPS CONSISTING OF SINGLE ADENINE RESIDUES CLOSED BY SHEARED A(DOT)A AND G(DOT)G PAIRS FORMED BY THE DNA TRIPLETS AAA AND GAG: SOLUTION STRUCTURE OF THE D(GTACAAAGTAC) HAIRPIN, NMR, 16 STRUCTURES===
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==Overview==
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The DNA undecamers GTACAAAGTAC (AAA 11-mer) and GTACGAGGTAC (GAG 11-mer) have been studied in solution by high-resolution NMR spectroscopy. Both duplexes form stable hairpins containing single deoxyadenosine loops and stems containing five base-pairs that are closed at the loop end by sheared AxA and GxC pairs, respectively. These molecules thus contain new AAA and GAG loop turn motifs. All protons, including the chiral H5'/H5" protons of the loop residues, were assigned using NOESY, DQF-COSY and heteronuclear 1H-31P COSY experiments. The backbone torsion angles were constrained using experimental data from NOE crosspeaks, three-bond 1H-1H coupling constants and four-bond 1H-31P coupling constants and four-bond 1H-31P coupling constants. The AAA and GAG 11-mers form similar structures in solution. The detailed structure of the AAA 11-mer was determined by the combined use of NMR, distance geometry and energy minimization methods. This structure exhibits good stacking of the loop adenosine base on the closing 5Ax7A sheared pair, with the 6A base stacking on the 5A base and the 6A deoxyribose stacking with the 7A base. All sugars in the AAA 11-mer hairpin adopt the typical DNA C2'-endo conformation and a sharp backbone turn occurs between residues 6A and 7A. This loop turn is brought about mainly by a change in the backbone phosphate torsion angles from zeta(g-) alpha(g-) to zeta(g+) alphat(g+) at the turn. The gamma torsion angle of residue 7A in the closing sheared pair also changes from gauche+ to trans. In Pu1NPu2 loop turns of the GCA, AAA and GAG types, the chemical shift of the H4' proton of the loop deoxyribose depends on the nature of Pu2; this reflects the stacking of the loop sugar on the Pu2 base and the different ring current effects of A or G in this position.
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(as it appears on PubMed at http://www.pubmed.gov), where 9000625 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9000625}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJH OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJH OCA].
==Reference==
==Reference==
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[[Category: Sheared a:a]]
[[Category: Sheared a:a]]
[[Category: Single residue loop]]
[[Category: Single residue loop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:35:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:15:53 2008''

Revision as of 16:15, 30 June 2008

Image:1bjh.png

Template:STRUCTURE 1bjh

HAIRPIN LOOPS CONSISTING OF SINGLE ADENINE RESIDUES CLOSED BY SHEARED A(DOT)A AND G(DOT)G PAIRS FORMED BY THE DNA TRIPLETS AAA AND GAG: SOLUTION STRUCTURE OF THE D(GTACAAAGTAC) HAIRPIN, NMR, 16 STRUCTURES

Template:ABSTRACT PUBMED 9000625

About this Structure

Full experimental information is available from OCA.

Reference

Hairpin loops consisting of single adenine residues closed by sheared A.A and G.G pairs formed by the DNA triplets AAA and GAG: solution structure of the d(GTACAAAGTAC) hairpin., Chou SH, Zhu L, Gao Z, Cheng JW, Reid BR, J Mol Biol. 1996 Dec 20;264(5):981-1001. PMID:9000625

Page seeded by OCA on Mon Jun 30 19:15:53 2008

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