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1bjw

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{{STRUCTURE_1bjw| PDB=1bjw | SCENE= }}
{{STRUCTURE_1bjw| PDB=1bjw | SCENE= }}
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'''ASPARTATE AMINOTRANSFERASE FROM THERMUS THERMOPHILUS'''
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===ASPARTATE AMINOTRANSFERASE FROM THERMUS THERMOPHILUS===
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==Overview==
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The three-dimensional structures of pyridoxal 5'-phosphate-type aspartate aminotransferase (AspAT) from Thermus thermophilus HB8 and pyridoxamine 5'-phosphate type one in complex with maleate have been determined by X-ray crystallography at 1.8 and 2.6 A resolution, respectively. The enzyme is a homodimer, and the polypeptide chain of the subunit is folded into one arm, one small domain, and one large domain. AspATs from many species were classified into aminotransferase subgroups Ia and Ib. The enzyme belongs to subgroup Ib, its sequence being less than 16% identical to the primary sequences of Escherichia coli, pig cytosolic, and chicken mitochondrial AspATs, which belong to subgroup Ia whose sequences are more than 40% identical and whose three-dimensional structures are quite similar with the active site residues almost completely conserved. The first X-ray analysis of AspAT subgroup Ib indicated that the overall and the active site structures are essentially conserved between the AspATs of subgroup Ia and the enzyme of subgroup Ib, but there are two distinct differences between them. (1) In AspAT subgroup Ia, substrate (or inhibitor) binding induces a large movement of the small domain as a whole to close the active site. However, in the enzyme of subgroup Ib, only the N-terminal region (Lys13-Val30) of the small domain approaches the active site to interact with the maleate. (2) In AspAT subgroup Ia, Arg292 recognizes the side chain carboxylate of the substrate; however, residue 292 of the enzyme in subgroup Ib is not Arg, and in place of Arg292, Lys109 forms a salt bridge with the side chain carboxylate. The thermostability of the enzyme is attained at least in part by the high content of Pro residues in the beta-turns and the marked increase in the number of salt bridges on the molecular surface compared with the mesophilic AspAT.
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(as it appears on PubMed at http://www.pubmed.gov), where 10029535 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10029535}}
==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:17:02 2008''

Revision as of 16:17, 30 June 2008

Image:1bjw.png

Template:STRUCTURE 1bjw

ASPARTATE AMINOTRANSFERASE FROM THERMUS THERMOPHILUS

Template:ABSTRACT PUBMED 10029535

About this Structure

1BJW is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structure of Thermus thermophilus HB8 aspartate aminotransferase and its complex with maleate., Nakai T, Okada K, Akutsu S, Miyahara I, Kawaguchi S, Kato R, Kuramitsu S, Hirotsu K, Biochemistry. 1999 Feb 23;38(8):2413-24. PMID:10029535

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