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1bp8

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{{STRUCTURE_1bp8| PDB=1bp8 | SCENE= }}
{{STRUCTURE_1bp8| PDB=1bp8 | SCENE= }}
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'''4:2:1 MITHRAMYCIN:MG2+:D(ACCCGGGT)2 COMPLEX'''
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===4:2:1 MITHRAMYCIN:MG2+:D(ACCCGGGT)2 COMPLEX===
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==Overview==
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Mithramycin and chromomycin, two antitumor drugs, each having an identical aglycone and nearly identical disaccharide and trisaccharide side chains, have differing binding properties to a small oligonucleotide, d(ACCCGGGT)(2) (M. A. Keniry et al., Journal of Molecular Biology, 1993, Vol. 231, pp. 753-767). In order to understand the forces that induce four mithramycin molecules to bind to d(ACCCGGGT)(2) instead of two drug molecules in the case of chromomycin, the structure of the 4:2:1 mithramycin: Mg(2+):d(ACCCGGGT)(2) complex was investigated by (1)H-nmr and restrained molecular dynamics. The resulting three-dimensional model showed that in order to accommodate the close approach of one neighboring mithramycin dimer, the inwardly directed CDE saccharide chain of the neighboring mithramycin dimer undergoes a conformational change such that the E saccharide no longer spans the minor groove but reorients so that the hydrophilic face of the E saccharides from the two dimers oppose each other. Two hydrogen bonds are formed between the hydroxyl groups of the two opposing E saccharide groups. The results are interpreted in terms of the differences in stereochemistry and functional group substitutions between mithramycin and chromomycin. A mithramycin dimer is able to self-associate on an oligonucleotide template because it has two hydroxyl groups on the same face of its terminal E saccharide. A chromomycin dimer is unable to self-associate because one of these hydroxyl groups is acetylated and the neighboring hydroxyl group has a stereochemistry that cannot permit close contact of the hydroxyl group with a neighbouring chromomycin dimer.Copyright 2000 John Wiley &amp; Sons, Inc.
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(as it appears on PubMed at http://www.pubmed.gov), where 10861371 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10861371}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BP8 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BP8 OCA].
==Reference==
==Reference==
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[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Oligonucleotide]]
[[Category: Oligonucleotide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:29:30 2008''

Revision as of 16:29, 30 June 2008

Image:1bp8.png

Template:STRUCTURE 1bp8

4:2:1 MITHRAMYCIN:MG2+:D(ACCCGGGT)2 COMPLEX

Template:ABSTRACT PUBMED 10861371

About this Structure

Full experimental information is available from OCA.

Reference

The three-dimensional structure of the 4:1 mithramycin:d(ACCCGGGT)(2) complex: evidence for an interaction between the E saccharides., Keniry MA, Owen EA, Shafer RH, Biopolymers. 2000 Aug;54(2):104-14. PMID:10861371

Page seeded by OCA on Mon Jun 30 19:29:30 2008

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