1btb

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[[Image:1btb.gif|left|200px]]
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{{STRUCTURE_1btb| PDB=1btb | SCENE= }}
{{STRUCTURE_1btb| PDB=1btb | SCENE= }}
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'''THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''
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===THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY===
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==Overview==
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We present the high-resolution solution structure and 13C assignments of wild-type barstar, an 89 amino acid residue polypeptide inhibitor of barnase, derived from heteronuclear NMR techniques. These were obtained from measurements on unlabeled, uniformly 15N- and 13C/15N-labeled, and 10% 13C-labeled barstar samples that have both cysteines (at positions 40 and 82) fully reduced. In total, 30 structures were calculated by hybrid distance geometry-dynamical simulated annealing calculations. The atomic rms distribution about the mean coordinate positions is 0.42 A for all backbone atoms and 0.90 A for all atoms. The structure is composed of three parallel alpha-helices packed against a three-stranded parallel beta-sheet. A more poorly defined helix links the second beta-strand and the third major alpha-helix. The loop involved in binding barnase is extremely well defined and held rigidly by interactions from the main body of the protein to both ends and the middle of the loop. This structure will be used to aid protein engineering studies currently taking place on the free and bound states of barstar and barnase.
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(as it appears on PubMed at http://www.pubmed.gov), where 8043574 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8043574}}
==About this Structure==
==About this Structure==
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1BTB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTB OCA].
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1BTB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTB OCA].
==Reference==
==Reference==
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[[Category: Lubienski, M J.]]
[[Category: Lubienski, M J.]]
[[Category: Ribonuclease inhibitor]]
[[Category: Ribonuclease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:56:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:41:31 2008''

Revision as of 16:41, 30 June 2008

Image:1btb.png

Template:STRUCTURE 1btb

THREE-DIMENSIONAL SOLUTION STRUCTURE AND 13C ASSIGNMENTS OF BARSTAR USING NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Template:ABSTRACT PUBMED 8043574

About this Structure

1BTB is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full experimental information is available from OCA.

Reference

Three-dimensional solution structure and 13C assignments of barstar using nuclear magnetic resonance spectroscopy., Lubienski MJ, Bycroft M, Freund SM, Fersht AR, Biochemistry. 1994 Aug 2;33(30):8866-77. PMID:8043574

Page seeded by OCA on Mon Jun 30 19:41:31 2008

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