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1bvo

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{{STRUCTURE_1bvo| PDB=1bvo | SCENE= }}
{{STRUCTURE_1bvo| PDB=1bvo | SCENE= }}
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'''DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA'''
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===DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA===
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==Overview==
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BACKGROUND: NF-kappa B/Rel transcription factors play important roles in immunity and development in mammals and insects. Their activity is regulated by their cellular localization, homo- and heterodimerization and association with other factors on their target gene promoters. Gambif1 from Anopheles gambiae is a member of the Rel family and a close homologue of the morphogen Dorsal, which establishes dorsoventral polarity in the Drosophila embryo. RESULTS: We present the crystal structure of the N-terminal specificity domain of Gambif1 bound to DNA. This first structure of an insect Rel protein-DNA complex shows that Gambif1 binds a GGG half-site element using a stack of three arginine sidechains. Differences in affinity to Dorsal binding sites in target gene promoters are predicted to arise from base changes in these GGG elements. An arginine that is conserved in class II Rel proteins (members of which contain a transcription activation domain) contacts the outermost guanines of the DNA site. This previously unseen specific contact contributes strongly to the DNA-binding affinity and might be responsible for differences in specificity between Rel proteins of class I and II. CONCLUSIONS: The Gambif1-DNA complex structure illustrates how differences in Dorsal affinity to binding sites in developmental gene promoters are achieved. Comparison with other Rel-DNA complex structures leads to a general model for DNA recognition by Rel proteins.
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{{ABSTRACT_PUBMED_10425685}}
==About this Structure==
==About this Structure==
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[[Category: Rel protein]]
[[Category: Rel protein]]
[[Category: Transcription factor]]
[[Category: Transcription factor]]
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Revision as of 16:47, 30 June 2008

Image:1bvo.png

Template:STRUCTURE 1bvo

DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA

Template:ABSTRACT PUBMED 10425685

About this Structure

1BVO is a Single protein structure of sequence from Anopheles gambiae. Full crystallographic information is available from OCA.

Reference

Structure of the specificity domain of the Dorsal homologue Gambif1 bound to DNA., Cramer P, Varrot A, Barillas-Mury C, Kafatos FC, Muller CW, Structure. 1999 Jul 15;7(7):841-52. PMID:10425685

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