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| - | [[Image:1bvw.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1bvw.png|left|200px]] |
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| | {{STRUCTURE_1bvw| PDB=1bvw | SCENE= }} | | {{STRUCTURE_1bvw| PDB=1bvw | SCENE= }} |
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| - | '''CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS'''
| + | ===CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS=== |
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| - | ==Overview==
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| - | The three-dimensional structure of the catalytic core of the family 6 cellobiohydrolase II, Cel6A (CBH II), from Humicola insolens has been determined by X-ray crystallography at a resolution of 1.92 A. The structure was solved by molecular replacement using the homologous Trichoderma reesei CBH II as a search model. The H. insolens enzyme displays a high degree of structural similarity with its T. reesei equivalent. The structure features both O- (alpha-linked mannose) and N-linked glycosylation and a hexa-co-ordinate Mg2+ ion. The active-site residues are located within the enclosed tunnel that is typical for cellobiohydrolase enzymes and which may permit a processive hydrolysis of the cellulose substrate. The close structural similarity between the two enzymes implies that kinetics and chain-end specificity experiments performed on the H. insolens enzyme are likely to be applicable to the homologous T. reesei enzyme. These cast doubt on the description of cellobiohydrolases as exo-enzymes since they demonstrated that Cel6A (CBH II) shows no requirement for non-reducing chain-ends, as had been presumed. There is no crystallographic evidence in the present structure to support a mechanism involving loop opening, yet preliminary modelling experiments suggest that the active-site tunnel of Cel6A (CBH II) is too narrow to permit entry of a fluorescenyl-derivatized substrate, known to be a viable substrate for this enzyme. | + | The line below this paragraph, {{ABSTRACT_PUBMED_9882628}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9882628 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9882628}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Cellulose degradation]] | | [[Category: Cellulose degradation]] |
| | [[Category: Glycoside hydrolase family 6]] | | [[Category: Glycoside hydrolase family 6]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:01:04 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:48:02 2008'' |
Revision as of 16:48, 30 June 2008
Template:STRUCTURE 1bvw
CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS
Template:ABSTRACT PUBMED 9882628
About this Structure
1BVW is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 A resolution., Varrot A, Hastrup S, Schulein M, Davies GJ, Biochem J. 1999 Jan 15;337 ( Pt 2):297-304. PMID:9882628
Page seeded by OCA on Mon Jun 30 19:48:02 2008
