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6vu2

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Current revision

M1214_N1 Fab structure

Structural highlights

6vu2 is a 2 chain structure with sequence from Human immunodeficiency virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.19Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The HIV-1 envelope (Env) undergoes conformational changes during infection. Broadly neutralizing antibodies (bNAbs) are typically isolated by using soluble Env trimers, which do not capture all Env states. To address these limitations, we devised a vesicular stomatitis virus (VSV)-based probe to display membrane-embedded Env trimers and isolated five bNAbs from two chronically infected donors, M4008 and M1214. Donor B cell receptor (BCR) repertoires identified two bNAb lineages, M4008_N1 and M1214_N1, that class-switched to immunoglobulin G (IgG) and IgA. Variants of these bNAbs reconstituted as IgA demonstrated broadly neutralizing activity, and the IgA fraction of M1214 plasma conferred neutralization. M4008_N1 epitope mapping revealed a glycan-independent V3 epitope conferring tier 2 virus neutralization. A 4.86-A-resolution cryogenic electron microscopy (cryo-EM) structure of M1214_N1 complexed with CH505 SOSIP revealed another elongated epitope, the V2V5 corridor, extending from V2 to V5. Overall, the VSVENV probe identified bNAb lineages with neutralizing IgG and IgA members targeting distinct sites of HIV-1 Env vulnerability.

VSV-Displayed HIV-1 Envelope Identifies Broadly Neutralizing Antibodies Class-Switched to IgG and IgA.,Jia M, Liberatore RA, Guo Y, Chan KW, Pan R, Lu H, Waltari E, Mittler E, Chandran K, Finzi A, Kaufmann DE, Seaman MS, Ho DD, Shapiro L, Sheng Z, Kong XP, Bieniasz PD, Wu X Cell Host Microbe. 2020 Apr 15. pii: S1931-3128(20)30188-8. doi:, 10.1016/j.chom.2020.03.024. PMID:32315598^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jia M, Liberatore RA, Guo Y, Chan KW, Pan R, Lu H, Waltari E, Mittler E, Chandran K, Finzi A, Kaufmann DE, Seaman MS, Ho DD, Shapiro L, Sheng Z, Kong XP, Bieniasz PD, Wu X. VSV-Displayed HIV-1 Envelope Identifies Broadly Neutralizing Antibodies Class-Switched to IgG and IgA. Cell Host Microbe. 2020 Apr 15. pii: S1931-3128(20)30188-8. doi:, 10.1016/j.chom.2020.03.024. PMID:32315598 doi:http://dx.doi.org/10.1016/j.chom.2020.03.024

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6vu2"

Categories: Human immunodeficiency virus | Large Structures | Kong X | Pan R

@@ Line 3: / Line 3: @@
 <StructureSection load='6vu2' size='340' side='right'caption='[[6vu2]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6vu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aids_virus Aids virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VU2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VU2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6vu2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VU2 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vu2 OCA], [http://pdbe.org/6vu2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vu2 RCSB], [http://www.ebi.ac.uk/pdbsum/6vu2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vu2 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vu2 OCA], [https://pdbe.org/6vu2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vu2 RCSB], [https://www.ebi.ac.uk/pdbsum/6vu2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vu2 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 </div>
 <div class="pdbe-citations 6vu2" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Aids virus]]
+[[Category: Human immunodeficiency virus]]
 [[Category: Large Structures]]
-[[Category: Kong, X]]
+[[Category: Kong X]]
-[[Category: Pan, R]]
+[[Category: Pan R]]
-[[Category: Antibody]]
-[[Category: Gp120]]
-[[Category: Immune system]]

6vu2

From Proteopedia

Current revision

M1214_N1 Fab structure

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

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