2vw9

From Proteopedia

(Difference between revisions)

Revision as of 11:47, 30 March 2022

Single stranded DNA binding protein complex from Helicobacter pylori

Structural highlights

2vw9 is a 3 chain structure with sequence from Campylobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SSB_HELPY] Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Single-stranded DNA (ssDNA)-binding protein (SSB) plays an important role in DNA replication, recombination, and repair. SSB consists of an N-terminal ssDNA-binding domain with an oligonucleotide/oligosaccharide binding fold and a flexible C-terminal tail involved in protein-protein interactions. SSB from Helicobacter pylori (HpSSB) was isolated, and the ssDNA-binding characteristics of HpSSB were analyzed by fluorescence titration and electrophoretic mobility shift assay. Tryptophan fluorescence quenching was measured as 61%, and the calculated cooperative affinity was 5.4x10(7) M(-1) with an ssDNA-binding length of 25-30 nt. The crystal structure of the C-terminally truncated protein (HpSSBc) in complex with 35-mer ssDNA [HpSSBc-(dT)(35)] was determined at a resolution of 2.3 A. The HpSSBc monomer folds as an oligonucleotide/oligosaccharide binding fold with a Y-shaped conformation. The ssDNA wrapped around the HpSSBc tetramer through a continuous binding path comprising five essential aromatic residues and a positively charged surface formed by numerous basic residues.

Single-stranded DNA-binding protein complex from Helicobacter pylori suggests an ssDNA-binding surface.,Chan KW, Lee YJ, Wang CH, Huang H, Sun YJ J Mol Biol. 2009 May 8;388(3):508-19. Epub 2009 Mar 13. PMID:19285993^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chan KW, Lee YJ, Wang CH, Huang H, Sun YJ. Single-stranded DNA-binding protein complex from Helicobacter pylori suggests an ssDNA-binding surface. J Mol Biol. 2009 May 8;388(3):508-19. Epub 2009 Mar 13. PMID:19285993 doi:10.1016/j.jmb.2009.03.022

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vw9"

@@ Line 3: / Line 3: @@
 <StructureSection load='2vw9' size='340' side='right'caption='[[2vw9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vw9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VW9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VW9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vw9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VW9 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vw9 OCA], [http://pdbe.org/2vw9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vw9 RCSB], [http://www.ebi.ac.uk/pdbsum/2vw9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vw9 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vw9 OCA], [https://pdbe.org/2vw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vw9 RCSB], [https://www.ebi.ac.uk/pdbsum/2vw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vw9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SSB_HELPY SSB_HELPY]] Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism (By similarity).
+[[https://www.uniprot.org/uniprot/SSB_HELPY SSB_HELPY]] Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 29: @@
 ==See Also==
-*[[Single stranded binding protein|Single stranded binding protein]]
 *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 == References ==

2vw9

From Proteopedia

Revision as of 11:47, 30 March 2022

Single stranded DNA binding protein complex from Helicobacter pylori

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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