2w2h

From Proteopedia

(Difference between revisions)

Revision as of 11:51, 30 March 2022

Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV

Structural highlights

2w2h is a 6 chain structure with sequence from Eiav and Equus caballus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TAT_EIAVY] Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The replication of many retroviruses is mediated by a transcriptional activator protein, Tat, which activates RNA polymerase II at the level of transcription elongation. Tat interacts with Cyclin T1 of the positive transcription-elongation factor P-TEFb to recruit the transactivation-response TAR RNA, which acts as a promoter element in the transcribed 5' end of the viral long terminal repeat. Here we present the structure of the cyclin box domain of Cyclin T1 in complex with the Tat protein from the equine infectious anemia virus and its corresponding TAR RNA. The basic RNA-recognition motif of Tat adopts a helical structure whose flanking regions interact with a cyclin T-specific loop in the first cyclin box repeat. Together, both proteins coordinate the stem-loop structure of TAR. Our findings show that Tat binds to a surface on Cyclin T1 similar to where recognition motifs from substrate and inhibitor peptides were previously found to interact within Cdk-cyclin pairs.

Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV.,Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M Nat Struct Mol Biol. 2008 Dec;15(12):1287-92. Epub 2008 Nov 23. PMID:19029897^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M. Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV. Nat Struct Mol Biol. 2008 Dec;15(12):1287-92. Epub 2008 Nov 23. PMID:19029897 doi:10.1038/nsmb.1513

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w2h"

@@ Line 3: / Line 3: @@
 <StructureSection load='2w2h' size='340' side='right'caption='[[2w2h]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Eiav Eiav] and [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W2H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Eiav Eiav] and [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2H FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [http://pdbe.org/2w2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [http://www.ebi.ac.uk/pdbsum/2w2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2h ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [https://pdbe.org/2w2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [https://www.ebi.ac.uk/pdbsum/2w2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2h ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TAT_EIAVY TAT_EIAVY]] Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).
+[[https://www.uniprot.org/uniprot/TAT_EIAVY TAT_EIAVY]] Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2w2h

From Proteopedia

Revision as of 11:51, 30 March 2022

Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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