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| | <StructureSection load='5k2y' size='340' side='right'caption='[[5k2y]], [[Resolution|resolution]] 2.41Å' scene=''> | | <StructureSection load='5k2y' size='340' side='right'caption='[[5k2y]], [[Resolution|resolution]] 2.41Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5k2y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K2Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5K2Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5k2y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K2Y FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k2x|5k2x]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.41Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">uspC, Rv2318 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k2y OCA], [https://pdbe.org/5k2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k2y RCSB], [https://www.ebi.ac.uk/pdbsum/5k2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k2y ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5k2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k2y OCA], [http://pdbe.org/5k2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k2y RCSB], [http://www.ebi.ac.uk/pdbsum/5k2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k2y ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P71894_MYCTU P71894_MYCTU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Besra, G S]] | |
| - | [[Category: Fullam, E]] | |
| - | [[Category: Futterer, K]] | |
| - | [[Category: Abc transporter]] | |
| | [[Category: Mycobacterium tuberculosis]] | | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Solute binding protein]] | + | [[Category: Besra GS]] |
| - | [[Category: Transport protein]] | + | [[Category: Fullam E]] |
| | + | [[Category: Futterer K]] |
| Structural highlights
Function
P71894_MYCTU
Publication Abstract from PubMed
Mycobacterium tuberculosis (Mtb), the aetiological agent of tuberculosis, has evolved to scavenge nutrients from the confined environment of host macrophages with mycobacterial ATP-binding cassette (ABC) transporters playing a key role in nutrient acquisition. Mtb-UspC (Rv2318) is the solute-binding protein of the essential transporter UspABC, one of four Mtb ABC transporters implicated by homology in sugar acquisition. Herein, we report the structural and functional characterization of Mtb-UspC. The 1.5 A resolution structure of UspC reveals a two subdomain architecture that forms a highly acidic carbohydrate-substrate binding cleft. This has allowed a distinct preference of Mtb-UspC for amino sugars as determined by thermal shift analysis and solution saturation transfer difference-NMR. Taken together our data support the functional assignment of UspABC as an amino-sugar transporter. Given the limited availability of carbohydrates within the phagosomal environmental niche during Mtb intracellular infection, our studies suggest that UspABC enables Mtb to optimize the use of scarce nutrients during intracellular infection, linking essentiality of this protein to a potential role in recycling components of cell-wall peptidoglycan.
Structural and functional analysis of the solute-binding protein UspC from Mycobacterium tuberculosis that is specific for amino sugars.,Fullam E, Prokes I, Futterer K, Besra GS Open Biol. 2016 Jun;6(6). pii: 160105. doi: 10.1098/rsob.160105. PMID:27335320[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fullam E, Prokes I, Futterer K, Besra GS. Structural and functional analysis of the solute-binding protein UspC from Mycobacterium tuberculosis that is specific for amino sugars. Open Biol. 2016 Jun;6(6). pii: 160105. doi: 10.1098/rsob.160105. PMID:27335320 doi:http://dx.doi.org/10.1098/rsob.160105
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