Journal:Acta Cryst D:S2059798320008475
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<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| + | We have developed a low-cost approach to optimally collect room temperature (RT) data from multiple crystals grown in microchips, using the counter-diffusion technique. We have shown the feasibility of obtaining 3D structural models at the highest attainable resolution, from crystals grown in microfluidic systems, and diffracted at RT (i.e. without harvesting individual crystals). Since sample handling and manipulation for cryo-protection are critical factors for X-ray structural determination, RT data collection of the three model proteins, glucose isomerase, lysozyme and thaumatin have allow us to determined their corresponding models at atomic resolution, near 1.0 ֳ…. | ||
| + | Furthermore, RT diffraction data can reveal motions crucial for catalysis, ligand binding, and allosteric regulation, not always accessible under standard cryogenic data collection. | ||
| + | The chips are fabricated by a combination of either OSTEMER-Kapton or OSTEMER-Mylar materials, both produce a sufficiently low scattering background to permit atomic resolution diffraction data collection at room temperature. The proposed system can be easily incorporated into a fully automatized workflow at any synchrotron beamline facilitating the collection of usersג€™ data with no-intervention required of the end-user. | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 14:00, 30 June 2020
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