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1c0w

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{{STRUCTURE_1c0w| PDB=1c0w | SCENE= }}
{{STRUCTURE_1c0w| PDB=1c0w | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE COBALT-ACTIVATED DIPHTHERIA TOXIN REPRESSOR-DNA COMPLEX REVEALS A METAL BINDING SH-LIKE DOMAIN'''
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===CRYSTAL STRUCTURE OF THE COBALT-ACTIVATED DIPHTHERIA TOXIN REPRESSOR-DNA COMPLEX REVEALS A METAL BINDING SH-LIKE DOMAIN===
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==Overview==
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The diphtheria toxin repressor (DtxR) is the prototype of a family of iron-dependent regulator (IdeR) proteins, which are activated by divalent iron and bind DNA to prevent the transcription of downstream genes. In Corynebacterium diphtheriae, DtxR regulates not only the expression of diphtheria toxin encoded by a corynebacteriophage, but also of components of the siderophore-mediated iron-transport system. Here we report the crystal structure of wild-type DtxR, a 226 residue three-domain dimeric protein, activated by cobalt and bound to a 21 bp DNA duplex based on the consensus operator sequence. Two DtxR dimers surround the DNA duplex which is distorted compared to canonical B -DNA. The SH3-like third domain interacts with the metal at site 1 via the side-chains of Glu170 and Gln173, revealing for the first time a metal-binding function for this class of domains. The SH3-like domain is also in contact with the DNA-binding first domain and with the second, or dimerization, domain. The DNA-binding helices in the first domain are shifted by 3 to 5 A when compared to the apo-repressor, and fit into the major groove of the duplex bound. These shifts are due to a hinge-binding motion of the DNA-binding domain with respect to the dimerization domains of DtxR. The third domain might play a role in regulating this hinge motion.
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{{ABSTRACT_PUBMED_10497029}}
==About this Structure==
==About this Structure==
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[[Category: Metal binding sh-like domain]]
[[Category: Metal binding sh-like domain]]
[[Category: Toxin repressor-dna complex]]
[[Category: Toxin repressor-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:03:25 2008''

Revision as of 17:03, 30 June 2008

Image:1c0w.png

Template:STRUCTURE 1c0w

CRYSTAL STRUCTURE OF THE COBALT-ACTIVATED DIPHTHERIA TOXIN REPRESSOR-DNA COMPLEX REVEALS A METAL BINDING SH-LIKE DOMAIN

Template:ABSTRACT PUBMED 10497029

About this Structure

1C0W is a Single protein structure of sequence from Corynebacterium diphtheriae. Full crystallographic information is available from OCA.

Reference

Crystal structure of a cobalt-activated diphtheria toxin repressor-DNA complex reveals a metal-binding SH3-like domain., Pohl E, Holmes RK, Hol WG, J Mol Biol. 1999 Sep 24;292(3):653-67. PMID:10497029

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