1i9f
From Proteopedia
(New page: 200px<br /> <applet load="1i9f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9f" /> '''STRUCTURAL CHARACTERIZATION OF THE COMPLEX ...) |
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| - | <applet load="1i9f" size=" | + | |
caption="1i9f" /> | caption="1i9f" /> | ||
'''STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE'''<br /> | '''STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the | + | INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the Rev response element RNA, as the natural Rev peptide does. The RSG-1.2 sequence has features incompatible with the helical structure of the bound Rev peptide, indicating that it must bind in a different conformation. RESULTS: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis. CONCLUSIONS: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state. |
==About this Structure== | ==About this Structure== | ||
| - | 1I9F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1I9F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA]. |
==Reference== | ==Reference== | ||
Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11358697 11358697] | Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11358697 11358697] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cho, H | + | [[Category: Cho, H S.]] |
[[Category: Frankel, A.]] | [[Category: Frankel, A.]] | ||
[[Category: Harada, K.]] | [[Category: Harada, K.]] | ||
| - | [[Category: Wemmer, D | + | [[Category: Wemmer, D E.]] |
[[Category: Zhang, Q.]] | [[Category: Zhang, Q.]] | ||
[[Category: peptide-rna complex]] | [[Category: peptide-rna complex]] | ||
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[[Category: solution structure]] | [[Category: solution structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:25 2008'' |
Revision as of 11:09, 21 February 2008
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STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE
Overview
INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the Rev response element RNA, as the natural Rev peptide does. The RSG-1.2 sequence has features incompatible with the helical structure of the bound Rev peptide, indicating that it must bind in a different conformation. RESULTS: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis. CONCLUSIONS: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state.
About this Structure
1I9F is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:11358697
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