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1c2n

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{{STRUCTURE_1c2n| PDB=1c2n | SCENE= }}
{{STRUCTURE_1c2n| PDB=1c2n | SCENE= }}
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'''CYTOCHROME C2, NMR, 20 STRUCTURES'''
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===CYTOCHROME C2, NMR, 20 STRUCTURES===
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==Overview==
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The solution structure, backbone dynamics and rotational diffusion of the Rhodobacter capsulatus cytochrome c2 have been determined using heteronuclear NMR spectroscopy. In all, 1204 NOE-derived distances were used in the structure calculation to give a final ensemble with 0.59(+/-0.08) A rms deviation for the backbone atoms (C, Calpha and N) with respect to the mean coordinates. There is no major difference between the solution structure and the previously solved X-ray crystal structure (1.07(+/-0.07) A rms difference for the backbone atoms), although certain significant local structural differences have been identified. This protein contains five helical regions and a histidine-heme binding domain, connected by a series of structured loops. The orientation of the helices provides an excellent sampling of angular space and thus allows a precise characterization of the anisotropic diffusion tensor. Analysis of the hydrodynamics of the protein has been performed by interpretation of the 15N relaxation data using isotropic, axially asymmetric and fully anisotropic diffusion tensors. The protein can be shown to exhibit significant anisotropic reorientation with a diffusion tensor with principal axes values of 1.405(+/-0.031)x10(7) s-1, 1.566(+/-0.051)x10(7) s-1 and 1.829(+/-0.054)x10(7) s-1. Hydrodynamic calculations performed on the solution structure predict values of 1.399x10(7) s-1, 1.500x10(7) s-1 and 1.863x10(7) s-1 when a solvent shell of 3.5 A is included in the calculation. The optimal orientation of the diffusion tensor has been incorporated into a hybrid Lipari-Szabo type local motion-anisotropic rotational diffusion model to characterize the local mobility in the molecule. The mobility parameters thus extracted show a quantitative improvement with respect to the model-free analysis assuming isotropic reorientation; helical regions exhibit similar dynamic properties and fewer residues require more complex models of internal motion. While the molecule is essentially rigid, a tripeptide loop region (residues 101 to 103) exhibits flexibility in the range of 20 to 30 ps, which appears to be correlated with the order in the NMR solution structure.
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{{ABSTRACT_PUBMED_9698552}}
==About this Structure==
==About this Structure==
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1C2N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C2N OCA].
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1C2N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C2N OCA].
==Reference==
==Reference==
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[[Category: Marion, D.]]
[[Category: Marion, D.]]
[[Category: Electron transport]]
[[Category: Electron transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:15:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:07:36 2008''

Revision as of 17:07, 30 June 2008

Image:1c2n.png

Template:STRUCTURE 1c2n

CYTOCHROME C2, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 9698552

About this Structure

1C2N is a Single protein structure of sequence from Rhodobacter capsulatus. Full experimental information is available from OCA.

Reference

Solution structure, rotational diffusion anisotropy and local backbone dynamics of Rhodobacter capsulatus cytochrome c2., Cordier F, Caffrey M, Brutscher B, Cusanovich MA, Marion D, Blackledge M, J Mol Biol. 1998 Aug 14;281(2):341-61. PMID:9698552

Page seeded by OCA on Mon Jun 30 20:07:36 2008

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