6v59
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of the diheme peroxidase BthA Y463M variant from Burkholderia thailandensis E264== | ==Crystal structure of the diheme peroxidase BthA Y463M variant from Burkholderia thailandensis E264== | ||
| - | <StructureSection load='6v59' size='340' side='right'caption='[[6v59]]' scene=''> | + | <StructureSection load='6v59' size='340' side='right'caption='[[6v59]], [[Resolution|resolution]] 1.59Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V59 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V59 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v59]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V59 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V59 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v59 OCA], [http://pdbe.org/6v59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v59 RCSB], [http://www.ebi.ac.uk/pdbsum/6v59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v59 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BTH_II1092 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v59 OCA], [http://pdbe.org/6v59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v59 RCSB], [http://www.ebi.ac.uk/pdbsum/6v59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v59 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BthA is a diheme enzyme that is a member of the bacterial cytochrome c peroxidase superfamily, capable of generating a highly unusual Fe(IV)Fe(IV) horizontal lineO oxidation state, known to be responsible for long-range oxidative chemistry in the enzyme MauG. Here, we show that installing a canonical Met ligand in lieu of the Tyr found at the heme of MauG associated with electron transfer, results in a construct that yields an unusually stable Fe(IV) horizontal lineO porphyrin at the peroxidatic heme. This state is spontaneously formed at ambient conditions using either molecular O2 or H2O2. The resulting data illustrate how a ferryl iron, with unforeseen stability, may be achieved in biology. | ||
| + | |||
| + | A Stable Ferryl Porphyrin at the Active Site of Y463M BthA.,Rizzolo K, Weitz AC, Cohen SE, Drennan CL, Hendrich MP, Elliott SJ J Am Chem Soc. 2020 Jul 1. doi: 10.1021/jacs.0c04023. PMID:32564595<ref>PMID:32564595</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6v59" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cytochrome c peroxidase 3D structures|Cytochrome c peroxidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Burta]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cohen | + | [[Category: Cohen, S E]] |
| - | [[Category: Drennan | + | [[Category: Drennan, C L]] |
| + | [[Category: Btha]] | ||
| + | [[Category: Diheme]] | ||
| + | [[Category: Heme]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Peroxidase]] | ||
Revision as of 11:36, 29 July 2020
Crystal structure of the diheme peroxidase BthA Y463M variant from Burkholderia thailandensis E264
| |||||||||||
Categories: Burta | Large Structures | Cohen, S E | Drennan, C L | Btha | Diheme | Heme | Oxidoreductase | Peroxidase
