6w26
From Proteopedia
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==Terpenoid Cyclase FgGS in Complex with Mg, Inorganic Pyrophosphate, and Imidazole== | ==Terpenoid Cyclase FgGS in Complex with Mg, Inorganic Pyrophosphate, and Imidazole== | ||
| - | <StructureSection load='6w26' size='340' side='right'caption='[[6w26]]' scene=''> | + | <StructureSection load='6w26' size='340' side='right'caption='[[6w26]], [[Resolution|resolution]] 2.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W26 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W26 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6w26]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W26 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W26 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w26 OCA], [http://pdbe.org/6w26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w26 RCSB], [http://www.ebi.ac.uk/pdbsum/6w26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w26 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FGRAMPH1_01T04331 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=229533 Fusarium graminearum])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w26 OCA], [http://pdbe.org/6w26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w26 RCSB], [http://www.ebi.ac.uk/pdbsum/6w26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w26 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Catalytic versatility is an inherent property of many enzymes. In nature, terpene cyclases comprise the foundation of molecular biodiversity as they generate diverse hydrocarbon scaffolds found in thousands of terpenoid natural products. Here, we report that the catalytic activity of the terpene cyclases AaTPS and FgGS can be switched from cyclase to aromatic prenyltransferase at basic pH to generate prenylindoles. The crystal structures of AaTPS and FgGS provide insights into the catalytic mechanism of this cryptic function. Moreover, aromatic prenyltransferase activity discovered in other terpene cyclases indicates that this cryptic function is broadly conserved among the greater family of terpene cyclases. We suggest that this cryptic function is chemoprotective for the cell by regulating isoprenoid diphosphate concentrations so that they are maintained below toxic thresholds. | ||
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| + | Discovery of the cryptic function of terpene cyclases as aromatic prenyltransferases.,He H, Bian G, Herbst-Gervasoni CJ, Mori T, Shinsky SA, Hou A, Mu X, Huang M, Cheng S, Deng Z, Christianson DW, Abe I, Liu T Nat Commun. 2020 Aug 7;11(1):3958. doi: 10.1038/s41467-020-17642-2. PMID:32769971<ref>PMID:32769971</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6w26" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Fusarium graminearum]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Christianson | + | [[Category: Christianson, D W]] |
| - | [[Category: Herbst-Gervasoni | + | [[Category: Herbst-Gervasoni, C J]] |
| + | [[Category: Lyase]] | ||
| + | [[Category: Terpene]] | ||
| + | [[Category: Terpenoid]] | ||
Revision as of 08:22, 20 January 2021
Terpenoid Cyclase FgGS in Complex with Mg, Inorganic Pyrophosphate, and Imidazole
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