6wk3
From Proteopedia
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==Engineered carbene transferase RmaNOD Q52V, putative nitric oxide dioxygenase from Rhodothermus marinus== | ==Engineered carbene transferase RmaNOD Q52V, putative nitric oxide dioxygenase from Rhodothermus marinus== | ||
| - | <StructureSection load='6wk3' size='340' side='right'caption='[[6wk3]]' scene=''> | + | <StructureSection load='6wk3' size='340' side='right'caption='[[6wk3]], [[Resolution|resolution]] 2.45Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WK3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WK3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wk3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhom4 Rhom4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WK3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WK3 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wk3 OCA], [http://pdbe.org/6wk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wk3 RCSB], [http://www.ebi.ac.uk/pdbsum/6wk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wk3 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rmar_2776 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=518766 RHOM4])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric_oxide_dioxygenase Nitric oxide dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.17 1.14.12.17] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wk3 OCA], [http://pdbe.org/6wk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wk3 RCSB], [http://www.ebi.ac.uk/pdbsum/6wk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wk3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | While biocatalysis is increasingly incorporated into drug development pipelines, it is less commonly used in the early stages of drug discovery. By engineering a protein to produce a chiral motif with a derivatizable functional handle, biocatalysts can be used to help generate diverse building blocks for drug discovery. Here we show the engineering of two variants of Rhodothermus marinus nitric oxide dioxygenase (RmaNOD) to catalyze the formation of cis- and tran- diastereomers of a pinacolboronate-substituted cyclopropane which can be readily derivatized to generate diverse stereopure cyclopropane building blocks. | ||
| + | |||
| + | Diversity-Oriented Enzymatic Synthesis of Cyclopropane Building Blocks.,Wittmann BJ, Knight AM, Hofstra JL, Reisman SE, Kan SBJ, Arnold FH ACS Catal. 2020 Jul 2;10(13):7112-7116. doi: 10.1021/acscatal.0c01888. Epub 2020 , Jun 4. PMID:33282460<ref>PMID:33282460</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wk3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Arnold | + | [[Category: Nitric oxide dioxygenase]] |
| - | [[Category: Knight | + | [[Category: Rhom4]] |
| + | [[Category: Arnold, F H]] | ||
| + | [[Category: Knight, A M]] | ||
| + | [[Category: Carbene transferase]] | ||
| + | [[Category: Globin]] | ||
| + | [[Category: Heme binding protein]] | ||
| + | [[Category: Metal binding protein]] | ||
Revision as of 10:55, 24 December 2020
Engineered carbene transferase RmaNOD Q52V, putative nitric oxide dioxygenase from Rhodothermus marinus
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