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6kkn

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Crystal structure of RuBisCO accumulation factor Raf1 from Anabaena sp. PCC 7120

Structural highlights

6kkn is a 1 chain structure with sequence from Nostoc sp. PCC 7120 = FACHB-418. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.85Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RAF1_NOSS1 A major RuBisCO chaperone. Acts after GroEL-GroES chaperonin to fold and/or assemble the large subunit of RuBisCO (ccbL, rbcL). Cooperates with RbcX in RbcL folding, plays the major role in assembly of dimers into RbcL(8)-Raf1(8) intermediate complexes. RbcS replaces Raf1, leading to holoenzyme formation.[HAMAP-Rule:MF_00856]^[1] In vitro acts as an antagonist to CcmM35, suggesting it might regulate RuBisCO condensation and decondensation.^[2]

Publication Abstract from PubMed

The folding and assembly of RuBisCO, the most abundant enzyme in nature, needs a series of chaperones, including the RuBisCO accumulation factor Raf1, which is highly conserved in cyanobacteria and plants. Here, we report the crystal structures of Raf1 from cyanobacteria Anabaena sp. PCC 7120 and its complex with RuBisCO large subunit RbcL. Structural analyses and biochemical assays reveal that each Raf1 dimer captures an RbcL dimer, with the C-terminal tail inserting into the catalytic pocket, and further mediates the assembly of RbcL dimers to form the octameric core of RuBisCO. Furthermore, the cryo-electron microscopy structures of the RbcL-Raf1-RbcS assembly intermediates enable us to see a dynamic assembly process from RbcL8Raf18 to the holoenzyme RbcL8RbcS8. In vitro assays also indicate that Raf1 can attenuate and reverse CcmM-mediated cyanobacterial RuBisCO condensation. Combined with previous findings, we propose a putative model for the assembly of cyanobacterial RuBisCO coordinated by the chaperone Raf1.

Molecular basis for the assembly of RuBisCO assisted by the chaperone Raf1.,Xia LY, Jiang YL, Kong WW, Sun H, Li WF, Chen Y, Zhou CZ Nat Plants. 2020 May 25. pii: 10.1038/s41477-020-0665-8. doi:, 10.1038/s41477-020-0665-8. PMID:32451445^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xia LY, Jiang YL, Kong WW, Sun H, Li WF, Chen Y, Zhou CZ. Molecular basis for the assembly of RuBisCO assisted by the chaperone Raf1. Nat Plants. 2020 May 25. pii: 10.1038/s41477-020-0665-8. doi:, 10.1038/s41477-020-0665-8. PMID:32451445 doi:http://dx.doi.org/10.1038/s41477-020-0665-8
↑ Xia LY, Jiang YL, Kong WW, Sun H, Li WF, Chen Y, Zhou CZ. Molecular basis for the assembly of RuBisCO assisted by the chaperone Raf1. Nat Plants. 2020 May 25. pii: 10.1038/s41477-020-0665-8. doi:, 10.1038/s41477-020-0665-8. PMID:32451445 doi:http://dx.doi.org/10.1038/s41477-020-0665-8
↑ Xia LY, Jiang YL, Kong WW, Sun H, Li WF, Chen Y, Zhou CZ. Molecular basis for the assembly of RuBisCO assisted by the chaperone Raf1. Nat Plants. 2020 May 25. pii: 10.1038/s41477-020-0665-8. doi:, 10.1038/s41477-020-0665-8. PMID:32451445 doi:http://dx.doi.org/10.1038/s41477-020-0665-8

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kkn"

@@ Line 3: / Line 3: @@
 <StructureSection load='6kkn' size='340' side='right'caption='[[6kkn]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kkn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KKN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kkn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KKN FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">all5250 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kkn OCA], [http://pdbe.org/6kkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kkn RCSB], [http://www.ebi.ac.uk/pdbsum/6kkn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kkn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kkn OCA], [https://pdbe.org/6kkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kkn RCSB], [https://www.ebi.ac.uk/pdbsum/6kkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kkn ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/RAF1_NOSS1 RAF1_NOSS1] A major RuBisCO chaperone. Acts after GroEL-GroES chaperonin to fold and/or assemble the large subunit of RuBisCO (ccbL, rbcL). Cooperates with RbcX in RbcL folding, plays the major role in assembly of dimers into RbcL(8)-Raf1(8) intermediate complexes. RbcS replaces Raf1, leading to holoenzyme formation.[HAMAP-Rule:MF_00856]<ref>PMID:32451445</ref>   In vitro acts as an antagonist to CcmM35, suggesting it might regulate RuBisCO condensation and decondensation.<ref>PMID:32451445</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Anabaena 7120]]
 [[Category: Large Structures]]
-[[Category: Chen, Y]]
+[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
-[[Category: Jiang, Y L]]
+[[Category: Chen Y]]
-[[Category: Kong, W W]]
+[[Category: Jiang YL]]
-[[Category: Xia, L Y]]
+[[Category: Kong WW]]
-[[Category: Zhou, C Z]]
+[[Category: Xia LY]]
-[[Category: Chaperone]]
+[[Category: Zhou CZ]]
-[[Category: Raf1]]
-[[Category: Rubisco]]

6kkn

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Current revision

Crystal structure of RuBisCO accumulation factor Raf1 from Anabaena sp. PCC 7120

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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