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| | ==Viral Ubiquitin from Autographa californica Nucleo Polyhedrosis Virus== | | ==Viral Ubiquitin from Autographa californica Nucleo Polyhedrosis Virus== |
| - | <StructureSection load='6kna' size='340' side='right'caption='[[6kna]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6kna' size='340' side='right'caption='[[6kna]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6kna]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Autographa_californica_mnpv Autographa californica mnpv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KNA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KNA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6kna]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_multiple_nucleopolyhedrovirus Autographa californica multiple nucleopolyhedrovirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KNA FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ac-v-ubi, LO84_035 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=307456 Autographa californica MNPV])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kna OCA], [https://pdbe.org/6kna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kna RCSB], [https://www.ebi.ac.uk/pdbsum/6kna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kna ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kna OCA], [http://pdbe.org/6kna PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kna RCSB], [http://www.ebi.ac.uk/pdbsum/6kna PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kna ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/UBIL_NPVAC UBIL_NPVAC] Viral ubiquitin that may play a role in the viral ubiquitination of nucleocapsid proteins to direct the egress of the nucleocapsid out of the nucleus, therefore mediating the formation of budded virus (BV).<ref>PMID:29142135</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6kna" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6kna" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[3D structures of ubiquitin|3D structures of ubiquitin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Autographa californica mnpv]] | + | [[Category: Autographa californica multiple nucleopolyhedrovirus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Das, R]] | + | [[Category: Das R]] |
| - | [[Category: Negi, H]] | + | [[Category: Negi H]] |
| - | [[Category: Reddy, P P]] | + | [[Category: Reddy PP]] |
| - | [[Category: Structure from cyana 2 1]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
UBIL_NPVAC Viral ubiquitin that may play a role in the viral ubiquitination of nucleocapsid proteins to direct the egress of the nucleocapsid out of the nucleus, therefore mediating the formation of budded virus (BV).[1]
Publication Abstract from PubMed
The Baculoviridae family of viruses encode a viral Ubiquitin gene. Though the viral Ubiquitin (vUb) is homologous to the host eukaryotic Ubiquitin (Ub), its preservation in the viral genome indicates unique functions that are not compensated by the host Ub. We report the structural, biophysical, and biochemical properties of the vUb from Autographa Californica Multiple Nucleo-Polyhedrosis Virus (AcMNPV). The packing of central helix alpha1 to the beta-sheet b1-b5 is different between vUb and Ub. Consequently, its stability is lower compared to Ub. However, the surface properties, ubiquitination activity, and the interaction with Ubiquitin binding domains are similar between vUb and Ub. Interestingly, vUb forms atypical polyubiquitin chain linked by lysine at the 54th position (K54), and the deubiquitinating enzymes are ineffective against the K54-linked polyubiquitin chains. We propose that the modification of host/viral proteins with the K54-linked chains is an effective way selected by the virus to protect the vUb signal from host DUBs.
-A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes.,Negi H, Reddy PP, Vengayil V, Patole C, Laxman S, Das R Biochem J. 2020 Jun 1. pii: 225104. doi: 10.1042/BCJ20200289. PMID:32478812[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Biswas S, Willis LG, Fang M, Nie Y, Theilmann DA. Autographa californica Nucleopolyhedrovirus AC141 (Exon0), a Potential E3 Ubiquitin Ligase, Interacts with Viral Ubiquitin and AC66 To Facilitate Nucleocapsid Egress. J Virol. 2018 Jan 17;92(3):e01713-17. PMID:29142135 doi:10.1128/JVI.01713-17
- ↑ Negi H, Reddy PP, Vengayil V, Patole C, Laxman S, Das R. -A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes. Biochem J. 2020 Jun 1. pii: 225104. doi: 10.1042/BCJ20200289. PMID:32478812 doi:http://dx.doi.org/10.1042/BCJ20200289
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