1jdk
From Proteopedia
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'''solution structure of lactam analogue (EDap) of HIV gp41 600-612 loop.'''<br /> | '''solution structure of lactam analogue (EDap) of HIV gp41 600-612 loop.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The conformational and immunological properties of different analogues | + | The conformational and immunological properties of different analogues corresponding to the 600-612 disulfide loop of the human immunodeficiency virus (HIV) gp41 glycoprotein envelope were studied. Fourteen analogues were designed and synthesised; namely, a series of seven analogues in which the disulfide bond was replaced by a lactam bridge and a series of seven analogues in which one residue of each analogue at a time, was replaced by its corresponding homologised alpha-amino acid (beta(3)-amino acid). In the case of the lactam analogues, the influence of the two possible CO-NH and NH-CO orientations of the lactam bridge as well as the size of the lactam ring was explored. The analogues were tested in ELISA with monoclonal antibodies raised against the 600-612 cyclic parent peptide as well as with sera from HIV-1 infected patients. A structural analysis of the parent and analogue peptides was carried out in dimethyl sulfoxide (DMSO-d(6)) using two-dimensional NMR techniques and molecular dynamics simulations. Comparison of the own conformation of the cyclic analogues with their either strong or weak reactivity with the antibodies reveals structural features that may be correlated with the antibody reactivity. Thus, a close structural similarity, particularly a characteristic orientation of the side-chains of residues Lys606, Leu607 and Ile608 in the loop, was found in certain beta(3)-analogues that were better recognised than the parent peptide by anti-peptide mouse monoclonal antibodies and patients' antibodies. |
==About this Structure== | ==About this Structure== | ||
| - | 1JDK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JDK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JDK OCA]. |
==Reference== | ==Reference== | ||
Structural and immunological characterisation of heteroclitic peptide analogues corresponding to the 600-612 region of the HIV envelope gp41 glycoprotein., Du AP, Limal D, Semetey V, Dali H, Jolivet M, Desgranges C, Cung MT, Briand JP, Petit MC, Muller S, J Mol Biol. 2002 Oct 25;323(3):503-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12381305 12381305] | Structural and immunological characterisation of heteroclitic peptide analogues corresponding to the 600-612 region of the HIV envelope gp41 glycoprotein., Du AP, Limal D, Semetey V, Dali H, Jolivet M, Desgranges C, Cung MT, Briand JP, Petit MC, Muller S, J Mol Biol. 2002 Oct 25;323(3):503-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12381305 12381305] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Briand, J | + | [[Category: Briand, J P.]] |
| - | [[Category: Cung, M | + | [[Category: Cung, M T.]] |
[[Category: Dali, H.]] | [[Category: Dali, H.]] | ||
[[Category: Desgranges, C.]] | [[Category: Desgranges, C.]] | ||
| - | [[Category: Du, A | + | [[Category: Du, A Phan Chan.]] |
[[Category: Jolivet, M.]] | [[Category: Jolivet, M.]] | ||
[[Category: Limal, D.]] | [[Category: Limal, D.]] | ||
[[Category: Muller, S.]] | [[Category: Muller, S.]] | ||
| - | [[Category: Petit, M | + | [[Category: Petit, M C.]] |
[[Category: Semetey, V.]] | [[Category: Semetey, V.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
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[[Category: peptidomimetics]] | [[Category: peptidomimetics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:24 2008'' |
Revision as of 11:21, 21 February 2008
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solution structure of lactam analogue (EDap) of HIV gp41 600-612 loop.
Overview
The conformational and immunological properties of different analogues corresponding to the 600-612 disulfide loop of the human immunodeficiency virus (HIV) gp41 glycoprotein envelope were studied. Fourteen analogues were designed and synthesised; namely, a series of seven analogues in which the disulfide bond was replaced by a lactam bridge and a series of seven analogues in which one residue of each analogue at a time, was replaced by its corresponding homologised alpha-amino acid (beta(3)-amino acid). In the case of the lactam analogues, the influence of the two possible CO-NH and NH-CO orientations of the lactam bridge as well as the size of the lactam ring was explored. The analogues were tested in ELISA with monoclonal antibodies raised against the 600-612 cyclic parent peptide as well as with sera from HIV-1 infected patients. A structural analysis of the parent and analogue peptides was carried out in dimethyl sulfoxide (DMSO-d(6)) using two-dimensional NMR techniques and molecular dynamics simulations. Comparison of the own conformation of the cyclic analogues with their either strong or weak reactivity with the antibodies reveals structural features that may be correlated with the antibody reactivity. Thus, a close structural similarity, particularly a characteristic orientation of the side-chains of residues Lys606, Leu607 and Ile608 in the loop, was found in certain beta(3)-analogues that were better recognised than the parent peptide by anti-peptide mouse monoclonal antibodies and patients' antibodies.
About this Structure
1JDK is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural and immunological characterisation of heteroclitic peptide analogues corresponding to the 600-612 region of the HIV envelope gp41 glycoprotein., Du AP, Limal D, Semetey V, Dali H, Jolivet M, Desgranges C, Cung MT, Briand JP, Petit MC, Muller S, J Mol Biol. 2002 Oct 25;323(3):503-21. PMID:12381305
Page seeded by OCA on Thu Feb 21 13:21:24 2008
Categories: Protein complex | Briand, J P. | Cung, M T. | Dali, H. | Desgranges, C. | Du, A Phan Chan. | Jolivet, M. | Limal, D. | Muller, S. | Petit, M C. | Semetey, V. | ACE | Cyclic peptide | Gp41 | Hiv | Lactam bond | Peptidomimetics
