6wpa
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Structure of AvaR1 bound to DNA half-site== |
| - | <StructureSection load='6wpa' size='340' side='right'caption='[[6wpa]]' scene=''> | + | <StructureSection load='6wpa' size='340' side='right'caption='[[6wpa]], [[Resolution|resolution]] 3.09Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6wpa]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avermitilis_MA-4680_=_NBRC_14893 Streptomyces avermitilis MA-4680 = NBRC 14893]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPA FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.09Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpa OCA], [https://pdbe.org/6wpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpa RCSB], [https://www.ebi.ac.uk/pdbsum/6wpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpa ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q82H41_STRAW Q82H41_STRAW] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Diffusible small molecule microbial hormones drastically alter the expression profiles of antibiotics and other drugs in actinobacteria. For example, avenolide (a butenolide) regulates production of avermectin, derivatives of which are used in the treatment of river blindness and other parasitic diseases. Butenolides and gamma-butyrolactones control production of pharmaceutically important secondary metabolites by binding to TetR family transcriptional repressors. Here, we describe a concise, 22-step synthetic strategy for the production of avenolide. We present crystal structures of the butenolide receptor AvaR1 in isolation, and in complex with avenolide, as well as AvaR1 bound to an oligonucleotide derived from its operator. Biochemical studies guided by the co-crystal structures enable identification of 90 new actinobacteria that may be regulated by butenolides, two of which are experimentally verified. These studies provide a foundation for understanding regulation of microbial secondary metabolite production, which may be exploited for the discovery and production of novel medicines. | ||
| + | |||
| + | Biochemical basis for the regulation of biosynthesis of antiparasitics by bacterial hormones.,Kapoor I, Olivares P, Nair SK Elife. 2020 Jun 8;9. pii: 57824. doi: 10.7554/eLife.57824. PMID:32510324<ref>PMID:32510324</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wpa" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Streptomyces avermitilis MA-4680 = NBRC 14893]] |
| + | [[Category: Kapoor I]] | ||
| + | [[Category: Nair SK]] | ||
| + | [[Category: Olivares PJ]] | ||
Current revision
Structure of AvaR1 bound to DNA half-site
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